Journal of Bioanalysis & Biomedicine

Torben Breindahl

Publications

• Research Article
  Quantification of Fentanyl in Human Serum by Column-Switching Liquid Chromatography and Tandem Mass Spectrometry
  Author(s): Torben Breindahl, Kristian Kjær Petersen, Mark Lillelund Rousing, Kirsten Andreasen, Lars Arendt-Nielsen and Peter HinderssonTorben Breindahl, Kristian Kjær Petersen, Mark Lillelund Rousing, Kirsten Andreasen, Lars Arendt-Nielsen and Peter Hindersson
  
  Analytical methods for pharmacokinetic studies with quantification of the highly potent anaesthetic drug fentanyl in serum must be rugged, sensitive, precise and accurate. To address these analytical demands and facilitate an automated approach to sample clean-up, a new method was developed for human serum using back-flush columnswitching and high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS). Prior to injection, protein precipitation in trichloroacetic acid was performed in each sample. Samples were diluted with internal standard (D5-fentanyl) in 10% (w/v) trichloroacetic acid, kept at 4oC for 20 min and centrifuged followed by injection of 40μL supernatant onto a BioTrap 500 MS extraction column. Using a time programmed six-port valve switch, the extracted drug was back-flushed onto a Zorbax SB-Aq analytical column, gradient eluted and finally.. Read More»
  DOI: 10.4172/1948-593X.1000052

  Abstract PDF