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Journal of Material Sciences & Engineering

ISSN: 2169-0022

Open Access

Trhlíková O


Czech Republic

Publications
  • Research Article
    Size-Exclusion Chromatography and Its Optimization for Material Science
    Author(s): Netopilík M and Trhlíková ONetopilík M and Trhlíková O

    A theoretical analysis of improving the separation power of size exclusion chromatography by decreasing flowrate is conveyed. The variance of the elution curves is larger than expected form estimated dispersity in molecular weight, Mw/Mn. The shape of the elution curves depends strongly on experimental conditions. When the experimental conditions are approaching those to the ideal separation, judged by statistical properties of the curves, both skew and excess kurtosis of the elution curves increase near the exclusion limit in accord with theoretical prediction. In analyses of polystyrene standards by size exclusion chromatography for polymers of molecular weight about thirty thousand and higher, the longitudinal diffusion is not important even at low flow-rates... Read More»
    DOI: 10.4172/2169-0022.1000358

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