Journal of Bioengineering & Biomedical Science

Valkova IP

Publications

• Research Article
  Development of Methods for Safe Application of Viral Vectors for GeneEngineering Manipulations
  Author(s): Iskra Ventseslavova Sainova, Valkova IP, Markova TZ and Nikolova EBIskra Ventseslavova Sainova, Valkova IP, Markova TZ and Nikolova EB
  
  Additional oncogene copy was transferred in normal cells, and additional tumor-suppression gene copy-in malignant cells, respectively, by transfection with the appropriate recombinant DNA-constructs, containing each one the respective gene. The results obtained were compared with the data from insertion of copy from the respective gene in prokaryotic bacteria strains E. coli, by analogical transfection with appropriate gene constructs. In application of low initial infections (high initial dilutions of viral suspensions, respectively) of eventually attenuated by many passages vaccine strains from the heterologous for mammals and mammalian cells avian viral species, only slight ultra-structural changes and signs of partially-formed immature viral particles in the cells, were established, but no cytopathic effect (CPE was observed). In freezing of the so inoculated mammalian cells after.. Read More»
  DOI: 10.4172/2155-9538.1000247

  Abstract PDF