Journal of Tissue Science and Engineering

Vera Grotheer

Publications

• Research Article
  The Effect of Aging and Culture Senescence on Fibroblast Proliferation and Osteogenic Differentiation
  Author(s): Vera Grotheer, Daniel Eckhardt, Julia Schulz, Olga Messel, Joachim Windolf and Christoph V SuschekVera Grotheer, Daniel Eckhardt, Julia Schulz, Olga Messel, Joachim Windolf and Christoph V Suschek
  
  Objective: The use of autologous cortical and cancellous bone remains the gold standard of bone-grafting. However, donor side morbidity, limited availability, and the risk of infections lead surgeons and researchers to seek suitable alternatives. Human fibroblasts are potent immunoregulatory cells with multipotent differentiation potential, which are easy to harvest and proliferate in vitro, which makes them attractive tools for bone tissue engineering. But for an autologous application in cell-based therapies, attention should be paid to the effect of donor age on differentiation potential. Culture senescence must also be considered, as some proliferation steps are necessary to obtain a sufficient cell number for therapeutic use. Methods: The results of this study reveal that an additional supplementation of insulin-like growth fac.. Read More»
  DOI: 10.37421/jtse.2019.10.222

  Abstract PDF