Journal of Bioprocessing & Biotechniques

Vijeta Sharma

Publications

• Review Article
  Application of System Biology Tools for the Design of Improved Chinese Hamster Ovary Cell Expression Platforms
  Author(s): Vijeta Sharma, Manjul Tripathi and KJ MukherjeeVijeta Sharma, Manjul Tripathi and KJ Mukherjee
  
  CHO cells have an impressive monopoly over other expression systems in the production of complex therapeutic proteins at large scale. There is thus a need to design superior cell lines with improved product quality and enhanced expression levels which requires an understanding of the cellular components and their interactions from a ‘systems’ point of view. With the emergence of critical ‘omics’ data sets for CHO cells which include transcriptomics, proteomics, metabolomics, fluxomics, and glycomics; some clarity has emerged in elucidating the global regulatory mechanisms that control protein over expression. Integrating this vast amount of information with bioprocess data can help point out significant targets for cellular modification that are required for hyper production. In mammalian systems, the information flow from genes to phenotype is mediated by comp.. Read More»
  DOI: 10.4172/2155-9821.1000284

  Abstract PDF