Biosensors & Bioelectronics

Villagrán MYS

Publications

• Research Article
  Evidence from Impedance Spectroscopy that Elevated Dopamine Reduces Mitochondrial Membrane Potential
  Author(s): Mitra RC, Villagrán MYS, Maric S, Wosik J, Zagozdzon-Wosik W and John H MillerMitra RC, Villagrán MYS, Maric S, Wosik J, Zagozdzon-Wosik W and John H Miller
  
  Background: Recent studies suggest that elevated dopamine concentrations reduce mitochondrial membrane potential, potentially contributing to neurological or other disorders. Changes in average membrane potential of suspended cells or organelles can be monitored in real time by measuring impedance of the suspension as a labelfree assay. Methods/Results: Here we report on measurements of frequency- and time-dependent electrical impedance of mitochondrial suspensions in the presence of various substrates, including dopamine. We find that, during succinateinduced state-2 respiration, addition of either trifluoromethoxy carbonylcyanide phenylhydrazone (FCCP), a known mitochondrial uncoupler (depolarizer), or dopamine at high concentrations cause similar increases in impedance, consistent with a drop in mitochondrial membrane potential. Conclusion: Our results provide further evidence that.. Read More»
  DOI: 10.4172/2155-6210.1000242

  Abstract PDF