Journal of Tissue Science and Engineering

VM Thomas

Publications

• Research Article
  In Vitro Production of Sheep Embryos in CR1aa Medium Supplemented with L-Ascorbic Acid
  Author(s): Dulam Sreenivas, DSVGK Kaladhar, Nagendra Sastry Yarla, VM Thomas, A PalniSamy, Varahala Rao Vadlapudi Varahala Rao Vadlapudi and R PreethiDulam Sreenivas, DSVGK Kaladhar, Nagendra Sastry Yarla, VM Thomas, A PalniSamy, Varahala Rao Vadlapudi Varahala Rao Vadlapudi and R Preethi
  
  In vitro embryo-production procedures developed for sheep have tremendously been improved from decades, but many factors influencing their efficiency still need to be investigated. The overall of this study showed the production of the sheep embryos till blastocyst stage from the ovaries of the slaughtered ewes for IVM, IVF of the oocytes and then IVC in the complex culture media like TCM-199, TCM-199-, TCM-199+ and CR1aa. The morula yield was significantly higher in the wheat peptone and BSA supplemented group. The nuclear maturation rates of ovine oocytes matured in FBS (69.15 ± 1.07) group was more compared to BSA (56.47 ± 0.73) and wheat peptone (35.26 ± 0.79). The nuclear maturation of oocytes was significantly higher in the FBS group when compared to BSA and wheat peptone supp.. Read More»
  DOI: 10.4172/2157-7552.1000131

  Abstract PDF