Discipline of Medical, Molecular and Forensic Sciences,
Perth WA 6150
Australia
Research Article
Immunohistochemical Detection of Haemoglobin Subunit Epsilon (HBE) in the Developing Mouse Placenta
Author(s): Layla H Al-Kinani, Flaminia Coiacetto, Claire R Sharp, Gabriele Rossi and Wayne K GreeneLayla H Al-Kinani, Flaminia Coiacetto, Claire R Sharp, Gabriele Rossi and Wayne K Greene
Introduction: Haemoglobin is a widely studied protein due to its important roles in physiology and pathology. Aberrant expression of haemoglobins, including primitive globins, have been reported in various sites and disease states and may have utility in some instances as diagnostic and/or prognostic markers. Despite this, robust detection of haemoglobin epsilon in the placenta during development by immunohistochemistry has not been well documented.Aim: To evaluate a polyclonal antibody against human haemoglobin subunit epsilon (HBE) by immunohistochemistry during primitive erythropoiesis in the developing mouse placenta.Methods and results: An immunohistochemistry protocol was developed using a commercially available antihuman haemoglobin subunit epsilon antibody on the mouse placenta at embr.. Read More»
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