Journal of Tissue Science and Engineering

Wilhelm K Aicher

Publications

• Research Article
  Labeling Mesenchymal Stromal Cells with PKH26 or VybrantDil Significantly Diminishes their Migration, but does not affect their Viability, Attachment, Proliferation and Differentiation Capacities
  Author(s): Alexandra Kelp, Tanja Abruzzese, Svenja Wöhrle, Viktoria Frajs and Wilhelm K AicherAlexandra Kelp, Tanja Abruzzese, Svenja Wöhrle, Viktoria Frajs and Wilhelm K Aicher
  
  Fluorescent dyes such as PKH26 and VybrantDil facilitate rapid and simple labeling of cells for later detection in various assays. But covering the cell surface with lipophilic fluorescent dyes may impair cellular functions. We therefore investigated the effects of PKH26 and VybrantDil on viability, proliferation, differentiation, attachment and migration of human mesenchymal stromal cells (MSCs) in vitro. To this end, MSCs were harvested from bone marrow of 12 individuals, expanded employing methods compliant to good manufacturing practice, and stained with PKH26 or VybrantDil. MSCs without label served as controls. The intensity of fluorescent staining as function of label and incubation time was investigated. Viability and proliferation were enumerated by cell counting. The osteogenic and adipogenic differentiations of MSCs were explored by cytochemical staining and transcript anal.. Read More»
  

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