Journal of Tissue Science and Engineering

Wilhelm K Aicher

Publications

• Research Article
  Adhesion to Extracellular Matrix Proteins can Differentiate Between Human Bone Marrow Derived Mesenchymal Stem Cells and Fibroblasts
  Author(s): Lorenzo P Roncoroni, Jan K Maerz, Brigitte Angres, Heiko Steuer, Karin Benz, Tanja Abruzzese, Melanie L Hart, Bernd Rolauffs, Gerd Klein, Dieter Stoll and Wilhelm K AicherLorenzo P Roncoroni, Jan K Maerz, Brigitte Angres, Heiko Steuer, Karin Benz, Tanja Abruzzese, Melanie L Hart, Bernd Rolauffs, Gerd Klein, Dieter Stoll and Wilhelm K Aicher
  
  Mesenchymal stem or stromal cells (MSC) contribute in vivo to wound repair and can be utilized for tissue regeneration. In contrast, fibroblasts may contribute to scar formation and may even hamper functional regeneration. Depending on the clinical application, MSC are sometimes attached to a scaffold to maintain the cells in the area of regeneration. We therefore screened for proteins that allow a preferential binding of MSC and avoid strong adherence of fibroblasts. The human MSC were isolated from bone marrow (bmMSC) or term placenta (pMSC). Synovial fibroblasts (SF) and dermal fibroblasts (DF) served as controls. In the first set of experiments, binding of bmMSC and SF to extracellular matrix (ECM) proteins was investigated by multiple substrate array (MSA®). From MSA® protein analyses 57 peptides with potential MSC-binding sites were selected and the binding of the cell.. Read More»
  DOI: 10.4172/2157-7552.S11-008

  Abstract PDF