Journal of AIDS & Clinical Research

William R. Abrams

Publications

• Research Article
  A Rapid, Self-confirming Assay for HIV: Simultaneous Detection of Anti- HIV Antibodies and Viral RNA
  Author(s): Zongyuan Chen, Hui Zhu, Daniel Malamud, Cheryl Barber, Yhombi Yvon Serge Ongagna, RubinaYasmin, Sayli Modak, Malvin N. Janal, William R. Abrams and Richard A. MontagnaZongyuan Chen, Hui Zhu, Daniel Malamud, Cheryl Barber, Yhombi Yvon Serge Ongagna, RubinaYasmin, Sayli Modak, Malvin N. Janal, William R. Abrams and Richard A. Montagna
  
  Objective: We developed a microfluidic system to simultaneously detect host anti-HIV antibodies and viral RNA in the same specimen in order to satisfy two important diagnostic criteria, especially within resource-limited settings. First, the system can detect acute HIV infection and allow immediate confirmation of a seropositive screening result by detection of HIV RNA. It also addresses the well-known "seroconversion window" during early HIV infection when antibodies are not yet detectable and viral loads are at their highest. Methods: We first developed and optimized two separate manual assays for the detection of host anti-HIV antibodies and viral RNA and then converted them to the microfluidic system. We optimized a commercially available serologic assay to run within the microfluidic device while we incorporated the isothermal LAMP assay to de.. Read More»
  DOI: 10.4172/2155-6113.1000540

  Abstract PDF