Journal of Tissue Science and Engineering

Wree A

Publications

• Research Article
  Chondrogenic Differentiation of Human Chondrocytes and Stem Cells in Different Cell Culture Systems Using IGF-1-Coupled Particles
  Author(s): Hiemer B, Krogull M, Zander K, Grüttner C, Bergschmidt P, Tischer T, Wree A, Bader R and Pasold JHiemer B, Krogull M, Zander K, Grüttner C, Bergschmidt P, Tischer T, Wree A, Bader R and Pasold J
  
  Various cell-based therapies use the transplantation of ex vivo cultured chondrocytes or stem cells to support repair of cartilage defects. Cell expansion in vitro is required prior to transplantation accompanied by cell dedifferentiation, resulting in unwanted fibrocartilage formation in vivo. Targeted application of growth factors during in vitro cultivation is intended to enhance chondrogenic differentiation of cells. In previous studies, collagen-based scaffolds enriched with silica particles coupled with the insulin-like growth factor (IGF) 1 were tested, concerning their suitability to increase the in vitro redifferentiation of human chondrocytes. Accordingly, in the present study chondrogenic differentiation potential of IGF-1-coupled particles was investigated using human chondrocytes cultured in scaffold-free spheroid pellet culture. Further, influence of IGF-1-coupled partic.. Read More»
  DOI: 10.4172/2157-7552.1000203

  Abstract PDF