Bolivarian Rep. of Venezuela
Research Article
Optimization of Polysaccharide Extraction from Polygonatum odoratum by Response Surface Methodology and Evaluation of its Antitumor Activity
Author(s): Zhan Xiao-ri, Xu Li-li, Xiong Feng, Li Xiao-lin, Yu Chun-na, Xie Tian and Li XiaZhan Xiao-ri, Xu Li-li, Xiong Feng, Li Xiao-lin, Yu Chun-na, Xie Tian and Li Xia
This work established a microwave-assisted procedure for polysaccharide extraction from Polygonatum odoratum. Response surface methodology was used to optimize microwave-assisted extraction parameters (extraction temperature, water-to-raw material ratio, microwave power, and extraction time) by implementing a three-level, fourvariable Box–Behnken experimental design in a single-factor investigation. Three-dimensional response surfaces were plotted by Design-Expert, and result indicated the absence of interaction effects of extraction temperature and microwave power. A polysaccharide yield of approximately 17.49% was obtained under the following optimized conditions: temperature of 57°C, microwave power of 300 W, extraction time of 10 min, and water-to-raw material ratio of 23:1. The results of preliminary in vitro antitumor activity tests showed that polysaccharides derived .. Read More»
DOI:
10.4172/2155-9821.1000292
Journal of Bioprocessing & Biotechniques received 3351 citations as per Google Scholar report