Veterinary Science & Technology

Yasuharu Mizuno

Publications

• Research Article
  Cytochrome P450 2D14 (CYP2D14) Gene Deletion Variants in the Japanese Black Cattle and Characterization of their Effects on Metoclopramide Pharmacokinetics
  Author(s): Kouko Hamamoto, Yasuharu Mizuno, Mayumi Kijima, Tsuyoshi Abe, Eiji Kobayashi, Masaki Kato, Norio Yamagishi and Kazuhisa FuruhamaKouko Hamamoto, Yasuharu Mizuno, Mayumi Kijima, Tsuyoshi Abe, Eiji Kobayashi, Masaki Kato, Norio Yamagishi and Kazuhisa Furuhama
  
  The frequency of cytochrome P450 2D14 (CYP2D14) genetic polymorphisms and their effects on CYP2D14- mediated metabolism have not been reported in cattle. Genetic polymorphisms of the CYP2D14 gene in 57 Japanese Black (JB) cattle were determined by polymerase chain reaction (PCR)-direct sequencing. Two types of CYP2D14 gene deletion variants (GD), GD1 and GD2, were observed in this study. The CYP2D6 deletion reportedly results in delayed metabolism of metoclopramide in human. To investigate the effects of CYP2D14 gene deletion in JB cattle, the GD1, GD2 and wild-type (WT) cattle were administered metoclopramide by an intravenous or oral route. Plasma concentrations of metoclopramide in GD1 cattle were significantly higher 24 hour (h) after intravenous administration (P<0.01) and within 1 h after oral administration of metoclopramide compared to levels in GD2 and WT cattle (P<0.01.. Read More»
  DOI: 10.4172/2157-7579.1000318

  Abstract PDF