Journal of Bioanalysis & Biomedicine

Yi K. Ng

Publications

• Research Article
  Simple Screening Method for Staurosporine in Bacterial Cultures using Liquid Chromatography-Tandem Mass Spectrometry
  Author(s): Amitha K. Hewavitharana, P. Nicholas Shaw, Yi K. Ng and John A. FuerstAmitha K. Hewavitharana, P. Nicholas Shaw, Yi K. Ng and John A. Fuerst
  
  Staurosporine has been shown to possess an array of important biological properties such as anti-fungal, anti-bacterial, antihypertensive and anti-cancer. Methods available to screen any plant or bacterial extract for this compound are lengthy and laborious. We present here a simple HPLC-MS-MS method for the highly selective identification of Staurosporine in various strains of the marine sponge-derived bacterium Salinispora. Gradient elution using acetonitrile/water/ammonium acetate was used to separate Staurosporine from the matrix and positive ion electrospray mass spectrometry was used for detection and confirmation. Presence of Staurosporine in bacterial extracts was confirmed by matching retention times and parent-daughter ion mass spectra (using Multiple Reaction Monitoring, MRM mode) of the standard Staurosporine with those of the bacterial extracts. Detection of Staurosporine.. Read More»
  DOI: 10.4172/1948-593X.1000001

  Abstract PDF