Molecular Biology: Open Access

Yinggang Luo

Publications

• Research Article
  Molecular Identification of Some Ghanaian Mushrooms Using Internal Transcribed Spacer Regions
  Author(s): Theresa Appiah, Christian Agyare and Yinggang LuoTheresa Appiah, Christian Agyare and Yinggang Luo
  
  Mushooms have recently attracted attention and are exploited for food and medicinal purposes. Accurate identification of mushrooms is key in utilizing them for the benefit of humans. However, morphological identification of mushrooms is time consuming, tedious and may be prone to error. DNA markers are quick and reliable tools that are useful in mushroom taxonomy. Thus this study confirmed the identity of six Ghanaian mushrooms using the internal transcribed spacer (ITS) sequences. The ribosomal DNA-ITS fragments of genomic DNA of six wild mushrooms were amplified using ITS1 and ITS4 primers. The amplicons were sequenced and data assembled and analyzed using Bio Edit. Basic Local Alignment Search Tool (BLAST) search was carried out using the National Center for Biotechnology Information (NCBI) database. The data obtained from the sequence alignment were used to plot a phylogenetic tre.. Read More»
  DOI: 10.4172/2168-9547.1000191

  Abstract PDF