IR Iran
Research Article
A Novel Technique for Harvesting Hepatic Vein Blood and Time Course of Posthepatic Reactive Oxygen Species after Liver Transplantation in Rats
Author(s): Changku Jia, Youke Chen and Honglei LiuChangku Jia, Youke Chen and Honglei Liu
Objective: To introduce a method for harvesting pure hepatic vein (HV) blood and to determine time course and peak level of posthepatic (HV blood) reactive oxygen species (ROS) after liver transplantation. Materials and methods: Blood was taken from HV and infrahepatic vena cava (IH-VC) for malondialdehyde (MDA) detection at different time points after transplantation.
Results: Pure posthepatic blood from HV was successfully obtained using the technique of IH-VC cannulation. This method guaranteed the harvesting of pure posthepatic blood from HV and pure peripheral blood from IH-VC. MDA concentration in HV elevated obviously with time and peaked at 1h after transplantation. MDA concentration at 1h was significantly higher than that at other time points except for 5 min. Furthermore, MDA conc.. Read More»
DOI:
10.4172/2161-0991.S1-008
Transplantation Technologies & Research received 223 citations as per Google Scholar report