Journal of Clinical & Medical Genomics

Yukihiko Ito

Publications

• Research Article
  Evaluation of ApoE Genotyping Using Saliva-Derived DNA
  Author(s): Takuya Kushioka, Makoto Ocho, Yukihiko Ito, Takafumi Yokokawa, Kei Yui and Yoshimitsu YamasakiTakuya Kushioka, Makoto Ocho, Yukihiko Ito, Takafumi Yokokawa, Kei Yui and Yoshimitsu Yamasaki
  
  Background: The single nucleotide polymorphism (SNP) regions (rs429358 and rs7412) that define the ApoE genotype have a high GC content, which makes genotyping difficult. Blood samples are mainly used for ApoE genotyping, and saliva has also been used in recent years. However, the equivalency of genotyping results using saliva samples has not been verified. Hence, we evaluated the equivalency of the genotyping results for saliva samples by two genotyping methods such as Sanger sequencing and restriction fragment length polymorphism (RFLP). Methods: Saliva and blood samples were collected from 51 subjects. The yield and quality of the extracted DNA were assessed. The regions around ApoE SNP were amplified by PCR, and genotyping was performed by Sanger sequencing and RFLP. Results: The mean concentration of saliva-derived .. Read More»
  DOI: 10.4172/2472-128X.1000149

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