Journal of Bioanalysis & Biomedicine

ZhongMao Guo

Publications

• Research Article
  Hepatic Endosome Protein Profiling in Apolipoprotein E Deficient Mice Expressing Apolipoprotein B48 but not B100
  Author(s): AnShu Chen, ZhongMao Guo, LiChun Zhou and Hong YangAnShu Chen, ZhongMao Guo, LiChun Zhou and Hong Yang
  
  Liver cells absorb apolipoprotein (Apo) B48-carrying lipoproteins in ApoE’s absence, albeit not as ef fi ciently as the ApoE-mediated process. Our objective was to identify differentially expressed hepatic endosome proteins in mice expressing ApoB48 but lacking ApoE and ApoB100 expression ( ApoE -/- /B48/48 ). We puri fi ed early and late endosomes from ApoE -/- /B48/48 and wild-type mouse’s livers. In ApoE -/- /B48/48 mouse’s hepatic endosomes, proteomic analysis revealed elevated protein levels of major urinary protein 6 (MUP), calreticulin, protein disul fi de isomerases (PDI) A1, and A3. These proteins are capable of interacting with lipids/lipoproteins and triggering receptor- mediated endocytosis. In addition, hepatic endosomes from ApoE -/- /B48/48 mice exhibited signi fi can.. Read More»
  DOI: 10.4172/1948-593X.1000031

  Abstract PDF