Medical Microbiology & Diagnosis

Department of Biology, Faculty of Sciences of Tunis, Laboratory of Parasitology, Military Hospital of Tunis, UR12DN03, Tunis, Tunisia

Publications

• Research Article   
  Diagnosis of Pneumocystis jirovecii Pneumonia by Real Time PCR: An Analysis of 10 Clinical Cases
  Author(s): Baccouchi Nawel*, Mtibaa Latifa, Souid Hana, Mehiri Emna, Slim Leila and Jemli Boutheina
  
  Introduction: Pneumocystis jirovecii Pneumonia (PCP) is a life-threatening disease in immunocompromised patients. The aim of this work is to demonstrate the contribution of molecular biology in diagnosis compared to conventional methods and we propose an adapted cut-off value for differentiating Pneumocystis colonization from infection using real-time PCR. Methodology: This was a prospective study enrolled from April 2015 to December 2018 at the Laboratory of parasitology of Military Hospital of Tunis. All pulmonary secretions samples were analyzed using: May-Grunwald-Giemsa (MGG) and Gomori Grocott Modified MUSTO Coloring Technology (GG). Optimization of conventional PCR and real-time PCR were accomplished. Results: During the study period, we collected 200 samples. The prevalence of the diseas.. Read More»
  DOI: 10.37421/2161-0703.2021.10.313

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