GET THE APP

..

Journal of Biodiversity & Endangered Species

ISSN: 2332-2543

Open Access

Chengwei Li

Department of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, China

Publications
  • Review   
    Bacterial Populations are Affected by DNA Extraction and Sampling Techniques
    Author(s): Chengwei Li*

    The amount and quality of DNA produced, as well as its appropriateness for subsequent PCR amplifications, varied significantly depending on the DNA extraction technique used. Quantitative PCR enumeration of microbial marker loci was used to further evaluate DNA extracts from nine extraction techniques that satisfied these initial quality standards. Depending on the DNA extraction technique utilised, the same rumen samples' absolute bacteria counts varied by more than 100-fold. Using 454 Titanium pyro sequencing, the apparent compositions of the archaeal, bacterial, ciliate protozoal, and fungal communities in similar rumen samples were evaluated... Read More»
    DOI: 10.37421/2332-2543.2022.10.446

    Abstract HTML PDF

Google Scholar citation report
Citations: 624

Journal of Biodiversity & Endangered Species received 624 citations as per Google Scholar report

Journal of Biodiversity & Endangered Species peer review process verified at publons

Indexed In

 
arrow_upward arrow_upward