Molecular Biology: Open Access

Department of Biotechnology, College of Natural and Computational Science, Wolkite University, Gurage,, Ethiopia

Publications

• Mini Review   
  A Rapid and Specific Loop Mediated Isothermal Amplification Molecular Detection Method; Current and Future Perspective
  Author(s): Degisew Yinur Mengistu*
  
  Loop-mediated isothermal amplification is a novel nucleic acid amplification method that relies on an auto-cycling strand displacement DNA synthesis, which is performed by Bst DNA polymerase. LAMP is a rapid and simple detection technique that can be used to diagnose several micro-organisms, including bacteria, viruses, parasites and fungi. It is a promising diagnostic and detection tool that could provide the results rapidly, and with high analytic sensitivity and specificity without the use of specialized instrumentation. Many researches and review articles have been published since the LAMP assay was established. However, there is lack of comprehensive review to cover LAMP primer design, mechanisms of amplification, detection methods, and applications together. This impeded and relegated its application for numerous types of disease diagnosis as a point care device. Therefore, this.. Read More»
  DOI: 10.4172/2168-9547.1000305

  Abstract HTML PDF