Veterinary Diagnostic Pathology, 638 South Fort Valley Road Fort Valley, Virginia, 22652, USA
Research Article
Histomorphometric Density Measurement of Bone Marrow Cellularity in Broiler Chickens
Author(s): Floyd D. Wilson*, Lanny Pace and Frederic J. Hoerr
Routine 5 μm H&E-stained histologic bone sections were utilized to investigate the capability of histomorphometry for determining bone marrow cellularity in commercial broiler chickens. Bone marrow mean pixel density was measured using the NIH ImageJ software program and the results for binary digital images were compared to corresponding results for actual bone marrow cell counts and cellular density scoring. A total of 220 bone marrow samples from femur and tibia obtained from broiler chickens, aged from hatch to 42 days were investigated. The average and standard deviation for all density measurements was 117 ± 26 pixels, for all cell counts 312 ± 124 cells per grid field and for all density scores 3.4 ± 0.8. Bone marrow density measurements from binary digital images and cellularity counts were highly correlated (correlation coefficient +0.83). Although.. Read More»
DOI:
10.37421/2157-7099.2022.13.656
Journal of Cytology & Histology received 2476 citations as per Google Scholar report