Department of Anesthesiology, School of Medicine, University of Texas Health Science Center San Antonio, 7703 Floyd Curl Drive, San Antonio, 78229 Texas, USA
Research Article
24-Hour Rat Hind Limb Preservation Using a 3D-Printed Subnormothermic Portable Machine Perfusion Device
Author(s): Rafael J Veraza, Jaclyn Merlo, Jerry Gelineau and Leonid Bunegin*
Background: Currently, severed limbs after combat or traumatic injuries are preserved with cold ischemic storage. However, this method maintains limb viability for no more than 12 hours. In this study, a new device, referred to as the Universal Limb Stasis System for Extended Storage (ULiSSES™), was used to maintain rodent skeletal muscle viability for 24 hours, after 4 hours of ambient temperature ischemia.
Methods: Hind-limbs from 5 Sprague Dawley rats were recovered and allowed to lie on a counter for approximately 4 hours at room temperature (19-24°C) to simulate delayed limb recovery. Limbs were then perfused for 24 hours using room temperature Krebs Henseleit solution. Arterial and venous pressure, flow, PaO2, PvO2, perfusate pH, and temperature were recorded hourly.
Results: Ambie.. Read More»
Transplantation Technologies & Research received 223 citations as per Google Scholar report
Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi 