Journal of Cytology & Histology

Division of Geriatrics, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

Publications

• Research Article   
  Enhanced Methods for Needle Biopsy and Cryopreservation of Skeletal Muscle in Older Adults
  Author(s): Cathy C Lee, Austin Hoang, David Segovia, Allen Herbst, Florian Barthelemy, Elizabeth Gibbs, Rachelle Crosbie, Stanley F Nelson, Carrie Miceli and Jonathan Wanagat*
  
  Human muscle biopsies are increasingly important for diagnosis, research, and to monitor therapeutic trials. We examined the use of a self-contained, vacuum-assisted biopsy system and a novel muscle freezing technique to improve, simplify, and standardize human muscle biopsy collection and cryopreservation in older adults. The VACORA vacuum-assisted biopsy system was deployed in muscle biopsies of 12 individuals ranging in age from 57 to 80 years. This office-based approach was well tolerated as it is minimally invasive, uses only local anesthetic, and has a quick recovery. To maximize biopsy sample quality and reproducibility, we developed a novel muscle sample freezing protocol. Fresh muscle biopsy samples were placed into readily available tissue cassettes followed by direct freezing in liquid nitrogen. After this modified snap freezing protocol, frozen muscle samples we.. Read More»
  DOI: 10.37421/jch.2020.11.553

  Abstract HTML PDF