Journal of Tissue Science and Engineering

Institute of Arizona Alzheimers Consortium, Phoenix, USA

Publications

• Research Article   
  Whole-Cell Dissociated Suspension Analysis in Human Brain Neurodegenerative Disease: A Pilot Study
  Author(s): Geidy E Serrano*, Jessica E Walker, Anthony J Intorcia, Michael J Glass, Richard A Arce, Ignazio S. Piras, Joshua S Talboom, Courtney M Nelson, Brett D Cutler, Lucia I Sue, Lih-Fen Lue, Matthew Huentelman and Thomas G Beach
  
  Biochemical analysis of human brain tissue is typically done by homogenizing whole pieces of brain and separately characterizing the proteins, RNA, DNA, and other macromolecules within. While this has been sufficient to identify substantial changes, there is little ability to identify small changes or alterations that may occur in subsets of cells. To effectively investigate the biochemistry of disease in the brain, with its different cell types, we must first separate the cells and study them as phenotypically defined populations or even as individuals. In this project, we developed a new method for the generation of Whole Cell Dissociated Suspensions (WCDS) in fresh human brain tissue that could be shared as a resource with scientists to study single human cells or populations. Characterization of WCDS was done in paraffin-embedded sections stained with H&E, and b.. Read More»
  DOI: 10.37421/2157-7552.2021.12.242

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