Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
Perspective
The Intricate Connections between Ketone Body Oxidation, Glycogen Stores, Glycolysis and Energy Metabolism in Cardiac Tissue
Author(s): Kadir Evans*
In the cardiac context, glucose and glycolysis play pivotal roles in supporting anaplerosis and potentially influencing the
oxidation of d-β-hydroxybutyrate (βHB). The presence of glycogen, serving as a reservoir for glucose, could also contribute
to anaplerosis. To delve into the intricate connections between glycogen content, βHB oxidation, glycolytic rates, and
their consequential impact on energy dynamics, an isolated rat heart model was employed. Hearts with high glycogen
(HG) and low glycogen (LG) levels were perfused with 11 mM [5-3H] glucose and/or 4 mM [14C] βHB to assess glycolysis
and βHB oxidation, respectively. Subsequently, freeze-clamping was carried out for glycogen and metabolomic analyses.
The ratio of free cytosolic [NAD+]/[NADH] and mitochondrial [Q+]/[QH2] was estimated using the lactate dehydrogenase
and succin.. Read More»
DOI:
10.37421/2684-494X.2023.8.68
Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi 