Molecular and Genetic Medicine

DreamTec Research Limited, Kowloon, Hong Kong, P.R. China

Publications

• Research Article   
  Expression of Human Epidermal Growth Factor in Escherichia coli by Intein Approach
  Author(s): Choi MC, Ma CHY, Lai ATL, Lin J and Kwong KWY*
  
  Different approaches are used to express recombinant proteins without the requirement of post-translational modification in Escherichia coli (E. coli). Though E. coli may have a drawback in producing endotoxin, its short division time and high expression of the final product are significant in making use as a host to produce various recombinant proteins. Inteins have been discovered in multiples microorganisms in facilitating the expression of homologous proteins and it has been used as one of the crucial tools for the production of recombinant proteins due to its unique feature in auto-excising its fusion partner, which also known as exteins. In this communication, we employed a well-established gp41-1 mini-intein to facilitate the expression of epidermal growth factor (EGF). The study revealed that though the epidermal growth factor cannot be excised from .. Read More»
  DOI: 10.37421/1747-0862.2020.14.S5

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