Journal of Forensic Medicine

Department of Chemistry, Saint Petersburg State University, St. Petersburg, Russia

Publications

• Short Communication   
  Connections of DNA with Lysozyme
  Author(s): Nikolay Noskov*
  
  AFM, ellipsometry, surface tensiometry, surface dilational rheology and infrared reflection-absorption spectroscopy (IRRAS) were used to investigate the interactions of DNA with lysozyme in the surface layer (AFM). Under a dispersed lysozyme layer, an aqueous subphase was injected with a concentrated DNA solution. In contrast to DNA interactions with a monolayer of a cationic synthetic polyelectrolyte, where the surface layer's optical properties changed quickly after DNA injection, the dynamic dilational surface elasticity almost did not change. This suggests that no continuous network of DNA/lysozyme complexes formed. The relatively quick increase in optical signals following a DNA injection behind a lysozyme layer suggests that diffusion regulates DNA penetration. The AFM images demonstrate the development of lengthy strands in the surface layer at low surfa.. Read More»
  DOI: 10.37421/2472-1026.2022.7.179

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