Journal of Forensic Medicine

Department of Chemistry, Saint Petersburg State University, St. Petersburg, Russia

Publications

• Short Communication   
  Interactions between DNA and Lysozyme
  Author(s): Nikolay Noskov*
  
  Various techniques including AFM, ellipsometry, surface tensiometry, surface dilational rheology, and infrared reflection-absorption spectroscopy (IRRAS) were employed to probe the interactions between DNA and lysozyme within the surface layer. In the experiments, a concentrated DNA solution was introduced into an aqueous subphase beneath a dispersed lysozyme layer. Notably, when compared to DNA's interactions with a monolayer of a cationic synthetic polyelectrolyte, the optical properties of the surface layer exhibited rapid changes following DNA injection, while the dynamic dilational surface elasticity remained relatively stable. This observation suggests the absence of a continuous network of DNA/lysozyme complexes. The swift increase in optical signals following DNA injection behind a lysozyme layer implies that DNA penetration is predominantly governed by diffusion. Furtherm.. Read More»
  DOI: 10.37421/2472-1026.2023.8.220

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