Global Journal of Technology and Optimization

Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, AR 72205, USA

Publications

• Mini Review   
  Cytogenetic and G-banding Molecular Biology in Human Immortal Endothelial Cells, Find New Translocations and Cryptic Aberrations
  Author(s): Rabiet Pathak*
  
  Cytogenetic, the study of chromosomes and their abnormalities, has been revolutionized by techniques like G-banding and molecular cytogenetic. These methodologies are crucial in understanding genetic disorders, identifying novel translocations and uncovering cryptic aberrations in various cell types, including Human Immortal Endothelial Cells (HIECs). This article explores the principles, applications and significance of G-banding and molecular cytogenetic in the context of HIECs. G-banding, a technique developed in the 1970s, involves staining chromosomes to create a characteristic banding pattern. The process begins with cell culture and metaphase arrest, often using colchicine or other mitotic inhibitors. Cells are then harvested and treated with hypotonic solution to swell them, making the chromosomes more accessible. Next, the cells are fixed and dropped o.. Read More»
  DOI: 10.37421/2229-8711.2024.15.386

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