Journal of Tissue Science and Engineering

Madhya Pradesh Council Science and Technology, 462003 Bhopal, Madhya Pradesh, India

Publications

• Research Article   
  High in Vitro Production of Secondary Metabolites or Regeneration of Catharanthus roseus Plant by using Plant Tissue Culture Technique
  Author(s): Stuti Bhatnagar*, Rajesh Saxena and Rashi Shrivastav
  
  Catharanthus roseus (periwinkle) is one of the most important medicinal and ornamental plants in the world. In this research, we will study about plant tissue culture of Catharanthus roseus and regeneration of plant to produce primary and secondary metabolites. An efficient protocol was standardized using axillary bud and shoots tip explants of Catharanthus roseus -an anticancer medicinal plant. In this investigation, periwinkle seeds, after sterilization were cultured on MS medium. Petiole segments of seedlings (4 day old) were su cultured to medium containing various concentrations of NAA accompanied with Kin and sub cultured t regenerate the callus and root. Callus and roots were obtained from petioles in some of treatments. The extracts of callus and roots from different treatments were analysed by spectrophotometer, TLC and HPLC with respect to the in.. Read More»
  DOI: 10.37421/2157-7552.2021.12.241

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