Cancer Science & Therapy

Professor Emeritus, Department of Surgery and Immunology, University of Toronto and the Toronto Hospital, Toronto, ON, Canada

Publications

• Research Article   
  Interaction of Immune Cells and Soluble Factors Which Contribute to Local and Metastatic Tumor Control within the Tumor Microenvironment
  Author(s): Reginald M. Gorczynski*
  
  Background and purpose: Transplantable immunogenic breast tumors (EMT6) in CD200RKO mice, but not wild-type (WT) animals, are cured of local and metastatic tumor growth following surgical resection and immunization with irradiated cells in adjuvant. By contrast tumor growth of the poorly immunogenic 4THM breast cancer was actually enhanced in CD200R1KO mice by comparison with WT mice. A novel two-phase culture system in which tumor invasion from a liquid phase occurs into a collagen gel containing bone marrow mesenchymal stromal cells (BMMSCs) was described which could recapitulate many of the differences observed in vivo between EMT6 and 4THM tumor cells. Invasion of tumor cells into the gel layer was monitored after collagenase digestion and culture of tumor cells at limiting dilution. Aliquots of the digest were assayed for cytokine levels in ELIS.. Read More»
  DOI: 10.37421/1948-5956.22.14.524

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