Medical Microbiology & Diagnosis

Department of Clinical and Chemical Pathology, Cairo University Specialized Pediatric Hospital (CUSPH), Cairo University, Egypt

Publications

• Research Article   
  Assessment of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of Mycoplasma Pneumoniae Infection in Paediatric University Hospital
  Author(s): Eiman M. Abdul Rahman, Amal Mohammed Sayed, Reham H.A. Yousef, Bassant Salah Saad Meligy and Samar Ahmed Mohammed Altohamy*
  
  Introduction: Community-acquired pneumonia (CAP) caused by M. pneumoniae affect 3%-10% of children. It is not possible to spot M. pneumoniae infection depending entirely on clinical signs and symptoms. Correct identification of M. pneumoniae infections is crucial to initiate proper antibiotic therapy. The role of culture is questionable as this organism is fastidious. Diagnostic accuracy of serology depends on the specimen collection time. IgM can usually be tracked within one week after the onset of clinical illness, followed by IgG two weeks later. Nucleic Acid Amplification Techniques (NAATs) are proved to be the “new gold standard”. Loop-Mediated Isothermal Amplification (LAMP) amplifies DNA of mycoplasma in less than an hour under isothermal conditions with the use of six primers in a single tube. The ampl.. Read More»
  DOI: 10.37421/2161-0703.2021.10.314

  Abstract HTML PDF