Department of Microbiology, Medical Laboratories Sciences, National Ribat University, Khartoum, Sudan
Research Article
Detection of Cytomegalovirus Glycoprotein B Genotypes among Early and Late Stages of HIV Infected Sudanese Patients
Author(s): Mahjoob O Mahjoob*, Shamson K Kafi, Abu Elgasim A Alsidig, Haitham E Elawad, Mustafa H Mustafa, Alteleb G Elnaim and Mohammed EH Ournasseir
The present research was conducted at the voluntary counseling and testing (VCT) center Khartoum, Sudan
during 2015-2016. This is a cross-sectional study. A sum of 194 individuals were recruited to take part in this research
(of them 96 were HIV patients in the first stage and 98 in the late stage of infection). Blood specimens were collected
from each subject. The specimens were screened for cytomegalovirus CMV-antibodies (IgM and IgG) using ELISA
(Acon Laboratories, Inc.), CMV-DNA was determined by Polymerase chain reaction PCR and CMV-gp (glycoprotein) B
genotyping was performed by Restriction fragment length polymorphism (RFLP). Results of this investigation showed
a significant prevalence of CMV infection. The results of HIV cases co-infected with CMV showed up that the disease
is higher among females than males and among cases in late-stage more than .. Read More»
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