Department of Medical Parasitology, Nemati Clinical Laboratory, Urmia, Iran
Research Article
Human Lymph Node Samples in Urmia, Iran Using PCR with GRA6 Gene from 2020 to 2021
Author(s): Mohammad Yousefzadeh* and Somayyeh Vakili Yekan
Background: Toxoplasmosis is one of the most common parasitic infections caused by a protozoan named Toxoplasma gondii. Humans
and carnivores can become infected by eating tissue cysts in row or semi-cooked meat or oocysts from cat feces. The aim of this study was
investigation of different genotypes of Toxoplasma gondii isolates from cat feces, lamb and human pathological samples to determine the
dominant types in Urmia city by amplifying GRA6 gen using PCR method.
Materials and Methods: Meat samples were collected from butchery shops of Urmia city by cutting a small piece of diaphragm muscle. DNA
extraction and PCR was done on these samples after being chopped. Stool samples from stray cats were collected from ruins and parks, and
oocysts were concentrated by ethyl acetate formalin and were stained with acid fast technique. Mi.. Read More»
Journal of Microbial Pathogenesis received 17 citations as per Google Scholar report