Dr, School of Biotechnology, IFTM University, Delhi Road (NH 24),244102 Moradabad, Uttar Pradesh, India
Research Article
High in Vitro Production of Secondary Metabolites or Regeneration of Catharanthus roseus Plant by using Plant Tissue Culture Technique
Author(s): Stuti Bhatnagar*, Rajesh Saxena and Rashi Shrivastav
Catharanthus roseus (periwinkle) is one of the most important medicinal and ornamental plants in the world. In this research, we will study about plant tissue culture of Catharanthus roseus and regeneration of plant to produce primary and secondary metabolites. An efficient protocol was standardized using axillary bud and shoots tip explants of Catharanthus roseus -an anticancer medicinal plant. In this investigation, periwinkle seeds, after sterilization were cultured on MS medium. Petiole segments of seedlings (4 day old) were su cultured to medium containing various concentrations of NAA accompanied with Kin and sub cultured t regenerate the callus and root. Callus and roots were obtained from petioles in some of treatments. The extracts of callus and roots from different treatments were analysed by spectrophotometer, TLC and HPLC with respect to the in.. Read More»
DOI:
10.37421/2157-7552.2021.12.241
Journal of Tissue Science and Engineering received 807 citations as per Google Scholar report