Journal of Integrative Oncology

Department of Dermatology, Massachusetts General Hospital, Harvard, USA

Publications

• Commentary   
  Novel Angiotens in Receptor Blocker, Azilsartan Induces Oxidative Stress and NFkB-Mediated Apoptosis in Hepatocellular Carcinoma cell line HEPG2
  Author(s): W. Robert Liu*
  
  Overexpression of Renin-Angiotensin System (RAS) and Nuclear Factor-Kappa B (NF-kB) has a key role in various cancers. Blockade of RAS and NF-kB pathway has been suggested to reduce cancer cell proliferation. This study aimed to investigate the role of angiotensin II and NF-kB pathway in liver Hepatocellular Carcinoma Cell Line (HepG2) proliferation by using azilsartan (as a novel Ag II antagonist) and Bay11-7082 (as NF-kB inhibitor). HepG2 cells were treated with different concentrations of azilsartan and Bay11- 7082. Cytotoxicity was determined after 24, 48, and 72 h by MTT assay. Reactive Oxygen Spices (ROS) generation and cytochrome c release were measured following azilsartan and Bay11-7082 treatment. Apoptosis was analyzed qualitatively by DAPI staining and quantitatively through flow cytometry methodologies and Bax and Bcl-2 mRNA and protein levels were assessed by real-time PC.. Read More»
  

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