Journal of Molecular Histology & Medical Physiology

Department of Physiology, Nutrition and Biomechanics, Swedish School of Sport and Health Sciences, Stockholm, Sweden

Publications

• Brief Report   
  Thrifty: Advancing Muscle Fiber Type Identification through High−Speed Precision Analysis
  Author(s): William Edman*
  
  Comprehensive insights into muscle physiology necessitate in-depth analyses of fiber types, yet the laborious nature of dissecting and typing individual fibers has posed significant challenges. This often limits investigations to a scant number of fibers from a handful of participants, casting doubts on the representativeness of both the fiber and participant populations. To surmount these obstacles and enable large-scale, fiber-specific studies, an innovative and rapid technique for high-throughput fiber typing of individually dissected fibers has been developed and named Thrifty (High-Throughput Immunofluorescence Fiber Typing). In the Thrifty approach, 400 fiber segments are affixed to microscope slides with a pre-printed grid system, subjected to antibody probing against myosin heavy chain (MyHC)-I and MyHC-II, and classified under a fluorescence microscope. .. Read More»
  DOI: 10.37421/2684-494X.2023.8.75

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