Cardiovascular Diseases & Diagnosis

Department of Clinical Pharmacology, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, P.R. China

Publications

• Research   
  α-Lipoic acid Prevents Angiotensin II-induced Endothelial Dysfunction via Antioxidant Effect and PI3K/Akt/eNOS Signaling Pathway
  Author(s): Nirmala Koju, Abdoh Taleb, Zhou Jifang, Lv Ge and Ding Qilong*
  
  Background: Oxidative stress is associated with endothelial dysfunction, the initial step in the pathogenesis of various cardiovascular disorders. Methods: HUVECs were divided into 3 groups including control group, model group (cells treated with 10-6 M Ang II for 24 hr) and treatment groups (cells treated with 0.1 mM, 0.25 mM, 0.5 mM and 1 mM ALA for 30 minutes and further incubated with 10-6 M Ang II for 24 hrs). The cytoprotective effect of ALA against Ang II was tested by MTT assay, ROS generation was evaluated using Di-chloroflourescein (DCFH) and Mitosox Red, NO, ET-1 and antioxidant enzymes (SOD, GPx, CAT) and IDH) activity were analyzed by their respective kits. Furthermore, Western blot was used to detect the protein expression of Akt, p-Akt, eNOS, p-eNOS, Nrf2, PGC1-α, Sirt3.. Read More»
  

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