Khare Soumya*, Chatterjee Tanushree, Gupta Shailendra and Patel Ashish
DOI: 10.37421/1948-593X.2023.15.379
Beta thalassemia is a disorder in which the body is unable to synthesise haemoglobin beta subunit due to deleterious mutations in the β-globin gene that results in underproduction of Adult Haemoglobin (HbA). Fetal Haemoglobin (HbF), which is composed of two α and two γ subunits, has been identified as a potential substitute for HbA with great clinical significance in β-thalassaemic patients. However, in the developmental stages, the expression of HbF is gradually minimized and overtaken by HbA. Our research found that the investigation of blood expression and its relationship to DEGs may aid in elucidating the role of these DEGs in beta thalassemia progression, and an RNA sequencing study indicated that the β globin gene is down regulated. There are 200 genes that are differently expressed in β thalassemia patients compared to healthy controls, as well as two key genes. KLF1 and MDM2 are two potential target genes for beta thalassemia patients that could be employed as diagnostic indicators. The differentially expressed genes include genes involved in heme biosynthesis, heme binding, erythrocyte homeostasis, iron ion binding, erythrocyte differentiation, gas transport and response to oxygen species metabolic processes, and other cellular processes. However, functional studies are needed to confirm their proposed relevance in beta thalassemia.
Md A Asaduzaman, Lufun Nahar, Md. Bakhtiar Lijon, Shahin Imran, Md Saidur Rhaman
Affinity Tags have been performed as the potential tools in the basic biological research field especially for production of recombinant protein and functional proteomics. Those affinity tags were wildly applied to simplify the purification of recombinant protein as well as differentiation of protein complex. Glutathione-S-Transferase (GST) tag has been extensively used in affinity chromatography for purification of fusion/recombinant protein to analysis of structure and function of protein, protein-protein interaction and to produce pharmaceutical product. In this review we describe the advantage of GST-tag in affinity chromatography technique as a method for inducible, high level protein expression and purification of recombinant protein. Recombinant protein which is expressed in a pGEX or pET vectors and that protein with GST-tag encoded at the NH2 - or COOH- region of genome sequence. There are some expression vectors which has different site to approve for unidirectional insertion of the coding region DNA, promoter, primers, antibiotic, Ori and GST-tag into pGEX vectors. By used reduced glutathione (GSH) during affinity chromatography the GST-tag with recombinant protein is eluted and stored it. Displacement of the GST-tag from the recombinant protein performed by protease enzyme for digestion which is purified by the application of another affinity technique.
Journal of Bioanalysis & Biomedicine received 3099 citations as per Google Scholar report
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