Y.N. SAI CHANDRA
Journal of Bioprocessing and Biotechniques commemorates its decade long service to the scientific community by consistently publishing peer-reviewed articles and tracking the progress and significant advancements in the field of biosensors. Ever since its inception in the year 2011, in addition to regular issue releases on a quarterly basis, this transdisciplinary journal is also releasing special issues and conference proceedings from time to time, thus comprehensively covering a wide range of topics and emerging challenges in bio techniques and bio processing. The journal focuses on application oriented research on biological, biochemical, biophysical and electronic principles with biological, biomedical and environmental relevance and utility. In this issue some of the recent and impactful research articles that were published by the journal will be discussed.
Rebecca Faschian, Steven Minden and Ralf Portner*
Lactic acid bacteria (LAB) like Lactococcus lactis ssp. lactis are used in food industry as well as for production of bacteriocins and optically pure lactic acid. Fixed-bed processes are highly productive but mathematical description is often complex. The aim of this work was to establish a mathematica model for process design. Biomass formation, lactose consumption and lactate production during start-up of fixed-bed cultures with immobilized L. lactis was investigated experimentally and described by a reaction kinetics model. Appropriate modeling and simulation of fixed-bed processes require biomass data. Therefore, a low-volume multiple fixed-bed reactor system (Multiferm) was used to investigate biomass formation of a L. lactis strain during the start-up phase of fixed-bed cultivation. The generation of data in parallel experiments was fast and easily compared to larger single reactor systems. Biomass data obtained from both fractions, retained and free suspended biomass, was used for modeling and simulation, together with data for lactose and lactate. The underlying Luedeking-Piret-like model structure was developed based on the results from suspension cultivations with the same strain. The fixed-bed system was described as perfusion culture with cell retention. For this, merely four additional parameters had to be defined to extend the suspension model to fixed-bed cultures. Experimental trends and steady states of both biomass fractions besides substrate and product could be described very well. Thus, this model could be used for process layout during process development..
Khalid S. Abdel-lateif1,4*, Ibrahim A. Maghrabi2,4 and Hany A. Eldeab3,4
The plant natural products are chemical compounds or substances produced by plants and have different activities or functions. These products exhibited several important biological activities such as antimicrobial, anti-allergic, antiinflammatory, antioxidant and free radical scavenging due to their rich content of compounds as polyphenols. The highly reactive oxygen species (ROS) generated during the metabolism can cause oxidative damage for biomolecules as RNA and DNA, resulting in cell cellular damage and death. It was shown that the antioxidant activities found in plant natural products could provide protective effects via inhibiting DNA oxidative damage. This review highlights some of important roles of the plant natural products as antioxidants and as antimicrobials.
Zhan Xiao-ri1,2, Xu Li-li1, Xiong Feng3, Li Xiao-lin2, Yu Chun-na1, Xie Tian2 and Li Xia3*
This work established a microwave-assisted procedure for polysaccharide extraction from Polygonatum odoratum. Response surface methodology was used to optimize microwave-assisted extraction parameters (extraction temperature, water-to-raw material ratio, microwave power, and extraction time) by implementing a three-level, fourvariable Box–Behnken experimental design in a single-factor investigation. Three-dimensional response surfaces were plotted by Design-Expert, and result indicated the absence of interaction effects of extraction temperature and microwave power. A polysaccharide yield of approximately 17.49% was obtained under the following optimized conditions: temperature of 57°C, microwave power of 300 W, extraction time of 10 min, and water-to-raw material ratio of 23:1. The results of preliminary in vitro antitumor activity tests showed that polysaccharides derived from P. odoratum could inhibit growth of A549 cells in a dose-dependent manner. Moreover, 4-400 μg/mL of these polysaccharides exerted no significant cytotoxicity to Panc-1 cells.
Ariana Vitale, Anita Mandal and Prabir K Mandal*
The purpose of this research was to study infant mortality and their rates in Duval County, Florida. Infant mortality rate is the estimate of infant deaths per 1,000 live births. The U.S. Infant Mortality Rate (IMR) currently ranks 27th among industrialized countries, with wide and persistent disparities by race, socioeconomic status, and geography. The objective of this research was to study infant mortality rates in each zip code in Duval County along with demographic information such as poverty, household income, prenatal care, and education. An analysis of the data collected was then used to establish whether there is a correlation between as poverty, household income, prenatal care, and education with infant mortality rates in zip codes with the highest and lowest infant mortality rates. The data for this research was gathered through the Florida Health department and nefloridacounts.org. The infant mortality rates and demographic information was sourced from the year 2014.
Sushantika Killer
Science is level headed and worth free, as it ought to be. It isn't the researcher's undertaking to make or change social, monetary, or political strategy. Target science is driven by interest in the normal world, mission of the utilizing establishment, and requests of the subsidizing that empower the examination. Researchers endeavor to comprehend and clarify the normal world, and innovation applies logical discoveries to the world. Public and logical discussions about biotechnology regularly seem, by all accounts, to be overwhelmed by total inverse perspectives.
Bioethanol is created principally from sugar and starch sourced from harvests, for example, sugar stick, wheat, and corn, which have a high convergence of sugar. Nonetheless, because these yields are additionally significant food sources, bioethanol created from them can fundamentally affect food costs and food security. Interestingly lignocellulosic biomass, buildups from wood or devoted energy crops is an option because there is no contest with food and creature feed creation. These materials are additionally less expensive than original biomass. Moreover, the utilization of lignocellulosic materials as fluid fills can support lessening ozone-depleting substance outflows. Lignocellulosic biomass is the wellspring of hexose and pentose sugars and is used in the production of bioethanol. In contrast to original biomass, the cellulose in the cell division of second-generation lignocellulosic substrates is encased in a hemicellulose and lignin network, making cellulose availability a key challenge in bioethanol production from such sources.
The source of ethanol is corn and other sugar-based crops, which has increased the pressure on food industries. The crops produced for consumption are now utilized in the formation of ethanol. The food processing industry is the second most waste-producing industry after household sewage. The solid and liquid waste released from the fruit industries can pose a vital contributor to land pollution. Processing of fruits can yield residues, which are in the form of pods, peels, pulp, stones, and seeds. Disposal of these residues without processing in landfills can further aggravate the situation by causing environmental pollution.
Md.Abdul Akhtar
In nature, there is harmony between microbes and non microorganisms. For example, the non-pathogenic microscopic organisms like nitrogen-fixing microorganisms ,eg.spirulina resides in the deep roots of the plant; lactobacillus in the milk helps in the change of milk to curd. There is a gigantic number of good microscopic organisms present in the human gut, which helps in food processing and assimilation. In this way, to disinfect our air with synthetic cleaners, we are possibly annihilating the non-pathogenic microorganisms too from the climate. Besides, these synthetic cleaners takes out sewage from the houses as polluted water, and contaminates the water bodies, air, and make the soil unfertile. For example, the clothing cleansers contain phosphate and nonbiodegradable branch-affixed alkyl sulphonates, which causes relentless froth
Mitochondria are extraordinary compartments (organelles) in our cells that are most popular for their job as forces to be reckoned with, as they breakdown food particles and turn out ATP, an atomic fuel for the remainder of the cell. Nonetheless, they complete numerous other significant organic cycles and are integral to the right working of the human cell. The mitochondrion has two films: an external layer, which is permeable for most atoms, and an inward layer, which is firmly fixed and has invaginations called cristae. Mitochondria can wire and separation and structure broad organizations in the cell that are exceptionally powerfu
Santanu Kumar*
Bioprocess technology is the modern use of natural cycles, including living cells and their components into substrates. The significant benefits of bioprocesses over conventional synthetic cycles are that they require gentle response conditions, are more explicit and proficient, and produce inexhaustible results (biomass). The advancement of recombinant DNA innovation has extended and expanded the capability of bioprocesses. A bioprocess is any interaction that utilizations complete living cells or their parts (e.g., microscopic organisms, catalysts, chloroplast) to acquire wanted items. This interaction is usually alluded to as Fermentation.
Spoornima Krishnan*, M Priya, H Jaya Ganga, V Malliga and K Yaazhini
DOI: 10.37421/2155-9821.2021.11.401
The plants are rich in anti-microbial agents for their anti-microbial activity. In this study, the powdered leaves extracts of Ixora coccinea and Datura metel were macerated and extracted with solvents in acetone, methanol and iso-propanol. The study was done to evaluate the anti-microbial property of leaf extracts of Ixora coccinea and Datura metel against the bacterial strain of Escherichia coli and Staphylococcus aureus by agar well diffusion method. From the antimicrobial activity assay, the best zone of inhibition formation li whereas Datura metel had shown good anti-bacterial activity against Staph of 11.8 ± 0.64 mm was observed in the case of Ixora coccinea against the bacterial strain of Escherichia coli Staphylococcus aureus by the inhibition zone formation of 15.3 ± 0.27 mm.
DOI: 10.37421/2155-9821.2021.11.405
Rupert D Holms* and Ravshan I Ataullakhanov
DOI: 10.37421/2155-9821.2021.11.403
Ezrin Peptide Therapy was invented in London and developed in Moscow over the previous three decades. Synthetic 14 amino acid peptides that mimic a receptor site in human ezrin, inhibit inflammation and amplify adaptive anti-viral immunity. Human Ezrin Peptide One (HEP1, Gepon) was registered for human use in the Russian Federation in 2001. The successful treatment with ezrin peptides of acute viral respiratory infection with pneumonia complications, led to testing of ezrin peptides as a potential COVID therapy. RepG3, a derivative peptide of HEP1, which has double the IL-6-expression inhibition activity, is being developed as a therapy for COVID and may also help to prevent SARS-CoV-2 re-infection.
DOI: 10.37421/2155-9821.2024.14.615
Anaerobic fungi are important members of the rumen microbiome, playing a crucial role in the degradation of plant biomass. However, their full potential in industrial applications, such as biofuel production, has not been fully realized. One approach to enhance the efficiency of anaerobic fungi is to create microbial consortiums with compatible partners. In this article, we discuss the use of in silico methods to identify potential microbial partners for anaerobic fungi, focusing on their metabolic interactions and the benefits of consortiums in biomass degradation.
DOI: 10.37421/2155-9821.2024.14.616
Purine derivatives are important compounds with diverse applications in the food and pharmaceutical industries. Traditional methods for their production are often complex and inefficient. In this article, we discuss a novel approach using one-pot multi-enzymatic reactions for the efficient synthesis of purine derivatives. We explore the advantages of this approach, its applications in the food and pharmaceutical industries, and future prospects for this technology. Purine derivatives are a class of organic compounds widely recognized for their significant roles in biological processes and their extensive applications in the food and pharmaceutical industries. They are key components of nucleotides, which are fundamental to DNA and RNA, and play crucial roles in cellular metabolism. The demand for purine derivatives is growing, driving the need for efficient and sustainable production methods. One-pot multi-enzymatic synthesis represents an innovative approach to produce these compounds efficiently and sustainably. This article explores the principles, methods, and applications of one-pot multi-enzymatic production of purine derivatives, focusing on their use in the food and pharmaceutical industries.
DOI: 10.37421/2155-9821.2024.14.617
The synthesis of retinyl laurate, a valuable nutraceutical compound, using lipase-catalyzed esterification is a promising approach. However, traditional methods suffer from low efficiency and yield. In this article, we propose a novel method that integrates artificial neural network (ANN) optimization with ultrasound support to enhance the lipase-catalyzed synthesis of retinyl laurate. We discuss the advantages of this approach, its potential applications in the food and pharmaceutical industries, and future research directions. Retinyl laurate is a compound derived from vitamin A (retinol) and lauric acid, known for its potential health benefits in skin care and nutrition. Traditional chemical synthesis of retinyl laurate is inefficient and involves the use of toxic reagents. Lipase-catalyzed esterification offers a more sustainable and efficient approach for the synthesis of retinyl laurate. However, the efficiency of this process can be further enhanced by optimizing reaction conditions. In this article, we propose a novel method that integrates ANN optimization with ultrasound support to improve the lipase-catalyzed synthesis of retinyl laurate.
DOI: 10.37421/2155-9821.2024.14.618
DOI: 10.37421/2155-9821.2024.14.619
DOI: 10.37421/2155-9821.2024.14.620
DOI: 10.37421/2155-9821.2024.14.621
DOI: 10.37421/2155-9821.2024.14.622
DOI: 10.37421/2155-9821.2024.14.623
DOI: 10.37421/2155-9821.2024.14.614
The production of ammonium and lactic acid is a major challenge in industrial animal cell culture, affecting cell growth, productivity, and product quality. Ammonium and lactic acid are by-products of metabolism, specifically from the breakdown of amino acids and glucose, respectively. Their accumulation can lead to a decrease in culture pH, cell viability, and overall productivity. Therefore, strategies to lower the production of these metabolites are crucial for optimizing cell culture processes. This article explores quick and effective methods to reduce the production of ammonium and lactic acid in industrial animal cell culture.
Journal of Bioprocessing & Biotechniques received 3351 citations as per Google Scholar report
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