Shinsuke Inoue, Keiji Sato, Shou Maruono, Haruto Nishida* and Tsutomu Daa
DOI: 10.37421/2157-7099.2024.15.752
Fluid Overload-Associated Large B-Cell Lymphoma (FO-LBCL) is a rare B-cell malignancy primarily found in body fluids without obvious mass formation or Human Herpes Virus type 8 (HHV-8) involvements. We report a case in which cell block specimens were useful for the diagnosis of FO-LBCL in the primary pericardial effusion of a human T-lymphotropic virus type 1 carrier patient. A 75-year-old man presented at our hospital complaining of fatigue and anorexia. Computed tomography revealed a massive pericardial effusion, but no mass lesions or enlarged lymph nodes. Analysis of the drained pericardial fluid revealed numerous solitary atypical cells with a high nuclear/cytoplasmic ratio and irregularly shaped nuclei of various sizes on Papanicolaou staining and blast-like and large atypical cells with multiple lobulated, clover-shaped and floating nuclei on Giemsa staining. Immunohistological staining of cell block specimens showed CD20 positivity and CD3 and HHV-8 negativity. The lesion was confined to the pericardial effusion and there was no obvious mass formation; hence, the patient was diagnosed with FO-LBCL. We observed the characteristic cytological features of FO-LBCL, such as clover-like or floating nuclei, in this case.
Rania AM Wahdan*, Mohamed S. Ali, Moustafa Mohamed Ibrahim Moustafa and Maha Zayed Mohammed Hassan
DOI: 10.37421/2157-7099.2024.15.753
Background: Ketamine has sedative, dissociative anesthetic and analgesic properties. Ketamine and its metabolites induced hepatic damage with chronic use for treatment of chronic pain. This study examined the effects of single intraperitoneal ketamine doseon adult male albino rats’ liver by applying histological and immunohistochemical methods.
Methods: After institutional animal research ethical committee approval, thirty healthy adult male albino rats were equally divided into three groups. Group I received Intraperitoneal (IP) saline in a similar volume to IP ketamine given in group II and III. Group II and Group III had 120 and 160 mg. Kg-1 body weight ketamine single intraperitoneal dose respectively. After 3 days’ rats were killed by decapitation and parts of their livers were processed and stained with Haematoxylin and Eosin (H and E) and Mallory trichrome for light microscope examination. Immunohistochemical examination for cyclooxygenase II enzyme (Cox II) was performed and the optical density of the reaction was calculated. Also, blood samples were taken from tail veins to measure Aspartate Transaminase (AST) and Alanine Transaminase (ALT) levels.
Results: H and E examination revealed normal hepatic architecture in rats’ liver that received normal saline (Group I). Hepatocytes were mildly affected in Group II. Marked affection of hepatocytes in group III with loss of normal hepatic architecture. Mild increase in connective tissue appeared after Mallory trichrome stain in group II, while more increase in group III was seen in the portal area. Also, liver enzyme Aspartate Transaminase (AST) level increased significantly in group II and III compared to group I. In addition, there was a significant increase in Alanine Transaminase (ALT) level only in group III when compared to group I and II.
Conclusion: It appears that single intraperitoneal doses of 120 mg and 160 mg ketamine can cause variable histological changes and damage in rats' liver.
DOI: 10.37421/2157-7099.2024.15.754
DOI: 10.37421/2157-7099.2024.15.755
DOI: 10.37421/2157-7099.2024.15.756
DOI: 10.37421/2157-7099.2024.15.757
DOI: 10.37421/2157-7099.2024.15.758
DOI: 10.37421/2157-7099.2024.15.759
DOI: 10.37421/2157-7099.2024.15.760
DOI: 10.37421/2157-7099.2024.15.761
DOI: 10.37421/2157-7099.2024.15.743
Cellular topography, the spatial distribution of cells within tissues, plays a crucial role in understanding tissue function and pathology. This article delves into the intricate cyto- and histopographic patterns of CPA3-positive cells, shedding light on their significance in various physiological and pathological contexts. CPA3, carboxypeptidase A3, serves as a marker for a distinct subset of cells with diverse functions in immunity, inflammation and tissue homeostasis. By elucidating the distribution of CPA3-positive cells across different tissues, this research provides valuable insights into their roles in health and disease.
DOI: 10.37421/2157-7099.2024.15.744
Type 3 transformation zones present unique challenges in cervical cytology interpretation due to their dynamic nature and potential for discrepancies in sampling. This article explores the clinical significance of negative cytology results within Type 3 transformation zones, highlighting diagnostic considerations and implications for patient management. Through a comprehensive review of current literature and clinical insights, we aim to elucidate the complexities of interpreting negative cytology in Type 3 transformation zones and provide guidance for healthcare professionals in optimizing cervical cancer screening strategies.
DOI: 10.37421/2157-7099.2024.15.745
Neurosurgical intraoperative histology techniques have significantly advanced in recent years, providing surgeons with real-time diagnostic information during surgical procedures. This abstract provides an overview of various techniques employed in intraoperative histology, including frozen section analysis, touch imprint cytology and rapid immunohistochemistry. These methods enable rapid pathological assessment of tissue samples, aiding in surgical decision-making and improving patient outcomes. Furthermore, emerging technologies such as stimulated Raman scattering microscopy and confocal microscopy offer high-resolution imaging capabilities, enhancing intraoperative tissue visualization. This abstract highlights the clinical relevance and utility of these techniques in guiding neurosurgical interventions, reducing operative time and minimizing the need for subsequent reoperations.
DOI: 10.37421/2157-7099.2024.15.746
DNA methylation, an epigenetic modification crucial for gene regulation, exhibits remarkable heterogeneity across different tissues, developmental stages and individuals. This mosaic-like pattern of methylation plays a pivotal role in cellular identity, differentiation and disease susceptibility. Understanding the intricacies of this heterogeneity offers insights into fundamental biological processes and opens avenues for therapeutic interventions. This review delves into recent advancements in elucidating the complex landscape of DNA methylation mosaic, highlighting its implications in health and disease.
DOI: 10.37421/2157-7099.2024.15.748
This comprehensive review delves into the intricate relationship between clinic-pathological features and immunohistochemical analysis. By synthesizing current research, it illuminates how immunohistochemistry enables a deeper understanding of disease processes, aiding in diagnosis, prognosis and therapeutic decision-making. From cancer to autoimmune disorders, the review navigates the diverse applications of this analytical tool, shedding light on its pivotal role in advancing clinical practice and enhancing patient care.
DOI: 10.37421/2157-7099.2024.15.750
DOI: 10.37421/2157-7099.2024.15.751
DOI: 10.37421/2157-7099.2024.15.742
DOI: 10.37421/2157-7099.2024.15.747
Thyroid malignancy presents a significant challenge in diagnosis due to its diverse histological presentations and the potential for overlapping features with benign lesions. Cytological examination plays a pivotal role in the initial assessment of thyroid nodules, guiding subsequent management decisions. This article provides an in-depth exploration of cytological insights into thyroid malignancy, highlighting diagnostic strategies to enhance accuracy and optimize patient care.
DOI: 10.37421/2157-7099.2024.15.749
Journal of Cytology & Histology received 2476 citations as per Google Scholar report
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