Skin and kidney are commonly affected in systemic lupus erythematosus (SLE) with similar molecular mechanisms. Although clinical indicators of renal injury in SLE are fairly uncontroversial, few biomarkers are reliable. The role of Micro-RNAs (micro-RNAs) in lupus nephritis (LN) pathogenesis has been investigated to help in early diagnosis. The aim of work is to evaluate microRNA132 expression in SLE Egyptian patients with and without nephritis and the relation between micro-RNA132 and its long non-coding gene SOX2 in both patients groups. This is a casecontrol study involving 100 SLE patients with and without LN (LN and non-LN groups), and 50 age-and sex-matched healthy controls were carried out to detect micro-RNA132 and SOX2 expression with quantitative Real-Time Polymerase chain reaction methods. The SLE disease activity index (SLEDAI) was assessed. SLEDAI was (2-7) and (4-14) in non-LN and LN respectively.micro-RNA132 expression was significantly increased in patient groups compared to controls (1-1) (P<0.001*) but increased in LN (1.3-7.8) compared to non-LN group (0.5-2.3)(P<0.001*).SOX2 was significantly decreased in patient groups compared to controls (1-1) (P<0.001*) more in LN (0.01-0.14) compared to non-LN group (0.05-0.45) (P<0.001*) There was negative correlation between miRNA132 and SOX2 expression in both patients groups r=-0.677 in non LN, r=--0.756 in LN, (P<0.001*).In conclusion,micro-RNA132 and SOX2 may play a role in SLE activity and helping in the early diagnosis of LN.
DOI: 10.37421/2155-9929.2024.15.652
DOI: 10.37421/2155-9929.2024.15.654
DOI: 10.37421/2155-9929.2024.15.655
DOI: 10.37421/2155-9929.2024.15.656
DOI: 10.37421/2155-9929.2024.15.658
DOI: 10.37421/2155-9929.2024.15.653
Ahamed Bilal
Ocimum species known for its traditional value,which is used as culinary herb and assigned as medicine and spice plant. We used molecular techniques to assess the genetic variability and relatedness of 20 accessions of three germplasm of Ocimum spp. (Ocimum gratissimum, Ocimum sanctum and Ocimum basilicum) collected from different places. DNA was isolated by fixing a sample in alcohol without using liquid nitrogen. Molecular markers were used to assess genetic diversity in basil (Ocimum L. spp., Lamiaceae). Varieties were analyzed through RAPD markers with five Inter-simple sequence repeats (ISSR) primers to determine the extent of molecular characterization. PCR amplification of these regions using a single primer yields multiple amplification products that can be used as a dominant multi locus marker system for the study of genetic variation in various organisms. and the result showed various gene expression with the ISSR primer than RAPD markers and we found a maximum number of unique alleles were observed in O. basillicum with ISSR primer (1, 5, 8, 9, 11, 12, 13, 14) which mentioned on primer synthesized table. Among the 15 ISSR primers tested, we have got bands in the size range of 300 bp to 850 bp. Our observations suggested that morphological variability and ISSR analysis could help in identifying genetic variations among different varieties of basil, help in plant improvement and develop a well-organized way to conserve the genetic wealth of basil varieties. DNA based markers like ISSR are more useful than the traditional morphological and biochemical markers. Genetic relationships were examined among thirty germplasm accessions belonging to five Ocimum species using RAPD and ISSR markers.
Molecular Biomarkers & Diagnosis received 2054 citations as per Google Scholar report
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