A Study on Advanced Surgical Neurooncology

Funjan AIA^*
*Correspondence: Funjan AIA, Department of Applied Biological Sciences, University of Science and Technology, Al-Ramtha, Jordan, Email:

Keywords

Helicase gene • Epstein Barr Virus • Brain tumor cells

Introduction

A scalpel procedure inside tumors cell of cerebellum (Medulloblastoma) and cerebrum (Glioma) create by Epstein Barr Virus 4 to 6 Helicase gene with highly density of plasmosomes estimated by 2000 CFU-Svedberg. In this issue a process of recognize of neurosurgery procedure will apply with for both diseases of brain tumors (Medulloblastoma and Glioma). For the fact that 4 to 6 genes of Helicase will act as scalpel and the plasmosomes actually act as seizure. One of the most important issue is to find a solution for tumor cells by destroying plasmodesmata between tumor cells by acting with Helicase gene type 5 of plasmodesmata. What will happen in this issue is to find mixing suitable solution inside the bonds of plasmodesmata of tumor cells which are actively high in mitotic division looks like a wire of electricity in its shape 2000 mcg and size 510 bp in its whole genome. In our catastrophe we manipulate 2 types of plasmodesmata cells 1-cell of Helicase gene from 4 to 6 genes of EBV 2-gene 5 of tumor cell plasmodesmata of plasmosomes of EBV. Other thing we manipulate 10% of Helicase of tissue culture animal cells in both human and mice.

Protocol

The protocol is found to create the same weather of Microinjection tumor cells inside brain of human and mice by advanced NANO EBV. What else inside cerebellum of mice-human brain and the other case inside cerebrum of same patients of the brain with both diseases. In this protocol the reputations is to have the same phenomena of Armed Epstein Barr Virus gene therapy at IJHS [1]. The Microinjection is built under these circumstances ; 1-7% of diluted solution of EBV serum when it centrifuged under 1000 USF 2-70% of albumin bovine serum 3-90% of tissue CSF 4-10% of mixture of Helicase gene (synthesized in myself funded laboratory) 5-7 to 12% of diluted Helicase genes of EBV of the same species of tumor cells when he do the same job as cancerous target issue 6-99% of highly density of plasmosomes (2000 Svedberg) 7-27% of diluted Armed Epstein Barr Virus Gene Therapy and this made from scientific IJHS by Ahmad Issa Ahmad Funjan article in October 2014 [1]. 8-tissue surface artifacts to reach tumor cells of both diseases (1 cm by 1 cube) in surface and density 9-10% of tissue culture Ligase gene to heal correct cells after neurosurgery procedure 10-gene 7 of human patients which is used to plasmodesma of tumor cells by plasmodesmatase in other words to make a helix loop helix between membrane of the undefected cells usually it equal=27 × 10⁸ bp and we dilute 5 units of it.

Materials and Methods

Plasmodesmata Epstein Barr Virus recognizing issue

Plasmodesmata is made by in our 2 processes of Helicase gene inside brain tumors cell and in tissue culture cells (in my lab). The first issue is downstream 4 to 6 genes of Helicase enzyme of Epstein Barr Virus immediate 2 regions of Helicase gene at inside brain tumor cells by affecting neurons process of plasmodesmata tumor cells. The Helicase gene will act as intermediate of weak plasmodesmata of tumor cells by adding 2 helix loop helix of Epstein Barr Virus immediate genes harmless 7 and 8 which of being removed by article of Ahmad Issa Ahmad Funjan at IJHS (Armed Epstein Barr Virus Gene Therapy Of Human Medulloblastoma Clinical Trial Phase-II 100% powerful & Fully Treated Vaccine), pp: 35-31 Issue 2, Vol. 2, October 2014 [1-5]. In this article we remove both harmless genes 7 & 8 from ATA-region of viron EBV then a catalysis procedure were made to destroy a tumor cells by the way of P-group addition. What we was from this procedure is to fully destroy plasmodesmata between tumor cells of both human and mice in the landscape and in the tissue culture level; the thought of idea is creating a fully length process of instability of tumor tissue and tumor cells inside brain of animal and human; what else is to find brilliant way in destroying all H-bonds and covalent bonds of plasma membrane of tumor cells (plasmodesmata destroying). The second process is used by enzyme linked Helicase gene 5 of Epstein Barr Virus which consider the best of gene that can Ligate EBV-genome to genes of tumor division also it can act as a seizure when it manipulated by the same article by Ahmad Issa Ahmad Funjan [1]. When we remove Hydrogenase enzyme by adding P-group which is 6000 mcg in shape and long chain in series with 5 bonds in helix loop helix. Then the protein enzyme like neurosurgeon of gene 5 of Epstein Barr Virus will doing of his job by fully treated removable of tumor cells inside the brain defected cells of the same species of human and animals by the needle of Exon of 5'-ATAGCTTTAGGGGGCTCT-'3 of gene targeted therapy. The Exon we remove the helix database of plasmodesmata of the same species tumor brain cells of cerebellum and cerebrum database of Medulloblastoma (neuroprimitive ectodermal tumor) and (Glial sheath of skull base tumor).

The Epstein Barr virus as clue of brain tumors neurosurgery

The development Neurosurgery of Epstein Barr Virus as a tool of brain tumors treatment at the medical field of human Glioma and Medulloblastoma is un recognized for about 10 years; amazing tool have been developed to face brain tumors surgery in public health; one of the most important is 4 to 6 Helicase gene when it surge in well done beauty process of Microinjection surgery inside tumor of the brain cells in both animals and human. The gene 5 of Helicase is strongly recommended by his EXON 5'-ATAGCTTTAGGGGGCTCT-'3 as a needle when he do his job inside tumor cells of ancient diseases. Other thing it is to behave as Armed tool in the process of gene therapy when phosphorilate tumor cell inside catalysis medium [1]. At this point no need for tumor surgery by endoscopic microscopy from this or that moment.

Cell culture of biopsy sample from animals and human brain tumors

The effect of neurosurgeon of Epstein Barr Virus helix loop helix of Phosphoatase of 20% of serum bovine albumin and 10% of Armed Epstein Barr Virus [1]; 25% of double strands of DNA of same Epstein Barr Virus (Arm 10); 12% of same species of 2 hybrid tissue between animals brain tumors (Medulloblastoma and Glioma) and human biopsy from surgical Neurooncology unit of Jordan university of science and technology university hospital; 12% of patients serum to fix if they defected with other tumors like Epstein Barr Virus defected tumors; 70% of Helicase gene 4 to 6 of EBV; plasmosomes with a highly density (2000 Svedberg); 95% of the whole genome of human gene 7 (it could be isolated by Reverse Transcriptase procedure of sequencing when we reach to his by western blotting it equal=27 × 10⁸ bp; 5 gene of Helicase of EBV with his Exon it reach to 510 bp and it is 5'-ATAGCTTTAGGGGGCTCT-'3 in its basis nitrogen; 5% of diluted CSF of same species of homo sapiens and red nude mice; 7–12% of liquid nitrogen when it froze to include destruction of Helicase genes before injected to tumors cell.

What we include from these and that basic unit is to providing the same weather of Microinjection for all species that we defected with same tumors brain of animal and/or human being.

The Microinjection procedure (intraparechymal inoculation)

In this case we prepare 75 needles for all species of being interested in order to reach 7 person of patients human disease with Glioma and Medulloblastoma; 73 were examine under electro Microsurgery Microscopy and the other were examine under tissue culture Microneurosurgery injection beside 700 of the red nude mice tissue culture by 2 hybridoma system; of that being we conclude that no need for hybridoma system with patients who hold the same disease of tumors of the brain.

Plasmodesmata of healthy tissue and brain tumors tissue

The big difference between 2 type of tissue the plasmodesmata is hugely big in healthy person and small in shape in defected tissue; other difference the plasmodesmata is hugely tense in between plasma membrane of healthcare person and very flexible in person with tumor disease; also the plasmosomes is highly active in health tissue and inactive in cancer cells; other thing plasmodesmata can react with gene 5 Exon as needle of plasma membrane of health tissue less than tumor plasma membrane of cancer tissue.

Results

Epstein Barr Virus toxicity monitoring

EBV shedding: The EBV antibody blood titers and body fluid cultures for Helicase gene from 4 to 6 especially neurosurgeon type 5 of Helicase genes used promoter type 7 of Epstein Barr Virus was negative in all patients clinical trial phase-III.

Local toxicity radiographic results: Early and delayed assessment of brain edema evaluated as described in methods, think of that no change in all patients with both tumor disease were recorded and no cerebellum/cerebrum edema was not observed.

Local toxicity histological findings: All patients with Medulloblastoma and Glioma at the time of recurrence and neurosurgeon gene type 5 of EBV treatment appear no symptoms at histological and MRI axial (T1 and T2 weighted images). In all examined specimens, there was no evidence of intraparechymal toxicity and the PCR analysis of brain sections at the Injection site is 7% in occurrence and 520 bp in parallel bands with these primers 1-reverse 5'-ACGCATTTCATGCCC-'3 and 2-forward 3'-GCCATCATCATTC-5' .

Serious adverse events: For adverse events of all patients no matter to be under medical control under university hospital, although no being side effects features were observed.

Quality of life, tumor progression monitoring, survival: In this study the Karnofsky performance score was observed and all patients have and give wealth care in fully life living and for Kaplan-Meier curve all patients were collect out of 00000 level of course of P=1.

Discussion

The articles were discovering a new therapeutic neurosurgeon gene type 5 from series of long genes of Epstein Barr Virus Helicase gene 4 to 6 chain. It was used to remove plasmodesmata of defected cells by Medulloblastoma and Glioma inside brain of cerebellum and cerebrum of animals and human patients diseases (PD) in this was the gene type 5 of Helicase of EBV when he destroy both covalent and weak bonds of tumor cells plasma membrane which responsible that of gene 7 of human being which have the ability to perform the plasmodesmata between plasma membrane of tumor cells [5-10].

Two procedures of manipulation prepare to face tumor cells of Medulloblastoma and Glioma by heavily attached of gene 5 of Helicase of what Epstein Barr Virus; being of thought the Armed flexible tiny helix loop helix plasmodesmata were surged by Epstein Barr Virus 5 gene long series of Helicase enzyme. 7 person were strongly attached (under surged) in a heavily amazing process by this neurosurgeon gene Exon. The other was attached by highly famous first article [1,2]. The percent of error in two attached were (0000000) and the other showed no signs. Finally all protocols, methods, instruments were in ISO 9001 and should be autoclaved.

Conclusion

The interim data of our clinical trial shows that the neurosurgeon gene 5 Exon of Epstein Barr Virus of 4 to 6 series of Helicase enzyme complex is safe to use in the clinical settings at the dose reported in our paper. The survival mean and quality of life of the patients observe a fully huge removable of cancer cells inside the brain with that of other Medulloblastoma/Glioma patients reported in the literature. Gene 5 Exon surgical neuroonclogist is one of the best therapeutic strategy for the treatment of brain tumors at the level of Medulloblastoma/Glioma neurosurgery. There is no doubt that harmless Epstein Barr Virus gene 5 Exon has the potential to become an important novel therapeutic option to treat both cerebrum and cerebellum tumors of the human brain. However, additional research on valvular heart tumors must be performed such as papillary fibroelastomas. In summary, the surgery tumors of human heart and brain using Ahmad Issa Ahmad Funjan Exon of Epstein Barr Virus Helicase gene 5 appear to be satisfactorily safe and advisable to be used at university hospital and general of neurosurgery departments at public and world levels.

Acknowledgements

We are sorry for Ethical regulation database to name some person defected with brain disease tumors. In that time many thanks for the first person in Hashemite Kingdome of Jordan (king Abdullah Bin AL Hussein-II) and the first lady in Jordan (queen Rania AL Abdullah – queen of Jordan) to support me and give mine spirit the service and efforts.

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