Beyond Antibiotics Exploring Innovative Antimicrobial Therapies

Stuart Bestow^*
*Correspondence: Stuart Bestow, Department of Biological Science Division, Autonomous Metropolitan University, Mexico City, Mexico, Email:

Keywords

Antibiotic resistance • Bacteriophage therapy • Nanotechnology

Introduction

Antibiotics have been a cornerstone of modern medicine, saving countless lives by effectively treating bacterial infections. However, their overuse and misuse have led to the emergence of antibiotic-resistant strains of bacteria, posing a significant threat to global health. In the quest to address this challenge, researchers are exploring innovative antimicrobial therapies that go beyond traditional antibiotics. This article delves into the cutting-edge developments in the field of antimicrobial therapies, from bacteriophage therapy to CRISPR-based approaches, highlighting the potential for these innovations to revolutionize the way we combat infectious diseases. Antibiotics revolutionized medicine in the 20^th century, ushering in an era where bacterial infections that were once deadly became easily treatable. However, the widespread use and misuse of antibiotics in human and animal healthcare, as well as in agriculture, have accelerated the development of antibiotic resistance. This phenomenon occurs when bacteria evolve mechanisms to survive exposure to antibiotics, rendering these drugs ineffective. The World Health Organization (WHO) has identified antibiotic resistance as one of the most significant global health threats. The rise of superbugs-bacteria resistant to multiple antibiotics has made it increasingly challenging to treat common infections, leading to prolonged illnesses, higher healthcare costs and an elevated risk of mortality [1].

Bacteriophages or phages for short are viruses that specifically target and infect bacteria. These viruses have been a part of Earth's micro biome for billions of years, coexisting with bacteria as natural predators. Bacteriophage therapy involves the use of these viruses to treat bacterial infections. Phages work by attaching to the surface of bacterial cells and injecting their genetic material into the host. This genetic material takes over the bacterial machinery, forcing the cell to produce more phages until it eventually bursts, releasing new phages that can infect neighboring bacteria. Importantly, bacteriophages are highly specific, targeting only the bacteria they are designed to attack, which minimizes collateral damage to beneficial bacteria in the body. One notable success story of bacteriophage therapy comes from the former Soviet Union, where it has been used for decades to treat bacterial infections. Recently, Western countries have started to explore the potential of bacteriophages as a viable alternative to antibiotics. Researchers are developing sophisticated methods to isolate and engineer phages to enhance their therapeutic potential. Nanotechnology offers another promising avenue for the development of innovative antimicrobial therapies. Nanoparticles, typically ranging in size from 1 to 100 nanometers, possess unique properties that make them effective at combating bacterial infections. Researchers are exploring various types of nanoparticles, including metallic nanoparticles, liposomes and dendrites, for their antimicrobial properties [2].

Literature Review

Silver nanoparticles, for example, have demonstrated potent antibacterial effects by disrupting bacterial cell membranes and interfering with cellular processes. Similarly, liposomes-tiny lipid vesicles-can be loaded with antimicrobial agents and targeted to specific bacterial strains. The ability to engineer nanoparticles with specific properties allows for precise targeting of pathogens while minimizing harm to surrounding tissues. Additionally, researchers are investigating the use of nanomaterials in coating medical devices and surfaces to prevent bacterial colonization and biofilm formation. This approach could reduce the risk of infections associated with the use of implants, catheters and other medical equipment. The human body is home to trillions of microorganisms, collectively known as the micro biome, which plays a crucial role in maintaining health and preventing infections. Probiotics, live microorganisms that confer health benefits when administered in adequate amounts, have gained attention as a potential tool in the fight against bacterial infections. Probiotics work by promoting the growth of beneficial bacteria in the body, which can help prevent the overgrowth of harmful bacteria. These beneficial bacteria may produce antimicrobial compounds, compete with pathogens for resources and modulate the host immune response. Probiotics have shown promise in preventing and treating various infections, including those of the gastrointestinal and urinary tracts [3].

Discussion

Furthermore, scientists are exploring ways to modulate the micro biome using precision medicine approaches. This involves tailoring interventions based on an individual's unique micro biome composition to promote a healthy microbial balance. By understanding the intricate relationships between different microbial species, researchers aim to develop targeted therapies that enhance the body's natural defenses against infections. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology, initially heralded for its revolutionary applications in gene editing, is now being explored as a potential tool in the fight against bacterial infections. CRISPR systems in bacteria serve as a form of immune defense, allowing the organisms to recognize and destroy the genetic material of invading viruses. Scientists are harnessing CRISPR technology to target and destroy specific bacterial pathogens. This approach involves designing CRISPR systems that specifically recognize the DNA sequences of harmful bacteria, effectively acting as programmable molecular scissors. Once deployed, these molecular scissors can precisely cut the bacterial DNA, rendering the pathogen unable to replicate and survive. While CRISPR-based antimicrobial strategies are still in the early stages of development, they hold immense promise for treating bacterial infections with unprecedented precision. The ability to tailor these therapies to specific pathogens could potentially minimize the risk of unintended consequences and reduce the emergence of resistance [4,5].

Peptides, short chains of amino acids, have gained attention as a class of molecules with potent antimicrobial properties. Naturally occurring antimicrobial peptides (AMPs) are a part of the innate immune system and are produced by various organisms, including humans. Researchers are exploring the therapeutic potential of both naturally occurring and synthetic peptides as a new class of antimicrobial agents. One advantage of peptides is their ability to target a broad spectrum of bacteria, including antibiotic-resistant strains. Some peptides work by disrupting bacterial cell membranes, while others interfere with essential cellular processes. Additionally, peptides can be engineered to enhance their stability, specificity and overall efficacy. Despite their promise, challenges such as limited stability and potential toxicity need to be addressed before peptide therapeutics can be widely used. Nevertheless, ongoing research and advancements in peptide engineering are bringing us closer to overcoming these obstacles and unlocking the full potential of peptides as antimicrobial agents. As the field of antimicrobial therapy advances, researchers are exploring the potential benefits of combining different approaches to enhance effectiveness and minimize the risk of resistance. Combination therapies involve using multiple antimicrobial agents simultaneously or sequentially to target different aspects of bacterial survival [6].

Conclusion

The rise of antibiotic resistance underscores the urgent need for innovative antimicrobial therapies that can effectively combat bacterial infections. The diverse approaches discussed in this article from bacteriophage therapy and nanotechnology to CRISPR-based strategies and peptide therapeutics represent the frontier of research in the field. As scientists continue to push the boundaries of our understanding of microbiology and molecular biology, these innovative therapies hold the potential to revolutionize the way we treat infections and mitigate the global threat of antibiotic resistance. However, it is crucial to approach these advancements with careful consideration of regulatory, ethical and societal implications to ensure responsible and sustainable progress in the fight against infectious diseases.

Acknowledgement

None.

Conflict of Interest

No potential conflict of interest was reported by the authors.

References

1. Schlievert, Patrick M. and Marnie L. Peterson. "Glycerol monolaurate antibacterial activity in broth and biofilm cultures." PloS one 7 (2012): e40350.

Google Scholar, Crossref, Indexed at

2. Mueller, Elizabeth A. and Patrick M. Schlievert. "Non-aqueous glycerol monolaurate gel exhibits antibacterial and anti-biofilm activity against Gram-positive and Gram-negative pathogens." PloS one 10 (2015): e0120280.

Google Scholar, Crossref, Indexed at

3. Nordström, Randi, Lina Nyström, Oliver CJ Andrén and Michael Malkoch, et al. "Membrane interactions of microgels as carriers of antimicrobial peptides." J Colloid Interface Sci 513 (2018): 141-150.

Google Scholar, Crossref, Indexed at

4. Comert Onder, Ferah, Selin Sagbas Suner, Nurettin Sahiner and Mehmet Ay, et al. "Delivery of small molecule EF2 kinase inhibitor for breast and pancreatic cancer cells using hyaluronic acid based nanogels." Pharm Res 37 (2020): 1-7.

Google Scholar, Crossref, Indexed at

5. Kłodzińska, Sylvia Natalie, Natalia Molchanova, Henrik Franzyk and Paul Robert Hansen, et al. "Biopolymer nanogels improve antibacterial activity and safety profile of a novel lysine-based α-peptide/β-peptoid peptidomimetic." Eur J Pharm Biopharm 128 (2018): 1-9.

Google Scholar, Crossref, Indexed at

6. Wang, Chunyan, Jaya Mallela, Ujjwala Sree Garapati and Sowndharya Ravi, et al. "A chitosan-modified graphene nanogel for noninvasive controlled drug release." Nanomed: Nanotechnol Biol Med 9 (2013): 903-911.

Google Scholar, Crossref, Indexed at