Nanogel For Breast Cancer Treatment

Masood F

Abstract

globally and is a main reason of mortality, thus requiring more therapeutic advancements. The epirubcin (EPI) is an anthracycline. However, the dose-related cardiotoxicity, neurotoxicity and myelosuppression are main problem associated with the use of available formulation of EPI. The poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) is considered as an ideal drug carrier due to its non-toxic, biodegradable and biocompatible nature. The blank and EPI loaded PHBV nanoparticles decorated with folic acid and polyethylene glycol were by prepared by nanoprecipitation method. Temperature sensitive hydrogel was prepared by addition of β-glycerophosphate disodium salt (β-GP) solution to chitosan (CS) solution. The CS/GP hydrogel demonstrated a rapid sol-to-gel transition at 37°C. The addition of folate grafted blank and drug loaded nanoparticles did not alter the gelation time of the resulting nanogels. The drug loading efficiency of EPI/FA-PEG-PHBV/CS nanogel was found to be 45.23%. About 60% of total encapsulated drug was released at pH 4 from EPI/FA-PEG-PHBV/CS nanogel during in-vitro release study. Blank FA-PEG-PHBV/CS nanogel did not affect the % viability of MCF7 breast cancer cell line, thus demonstrating their non-toxicity and biocompatibility. The EPI/FA-PEG-PHBV/CS nanogel significantly inhibited the viability and proliferation of cancer cells in comparison to equivalent amount of free drug.

Introduction

Different mechanisms in cancer cells become proof against one or a lot of chemotherapeutics is thought as multidrug resistance (MDR) that hinders therapy effectiveness. Potential factors for MDR includes increased drug detoxification, faded drug uptake, increased intracellular nucleophiles levels, increased repair of drug induced DNA injury, over expression of drug transporter like P-glycoprotein(P-gp), multidrug resistance-associated proteins (MRP1,MRP2), and carcinoma resistance supermolecule (BCRP). New chemotherapeutical drug delivery systems are developed tocombat drug resistance and multidrug resistance. Nanogel ar getting used to deliver medicine a lot of effectively in cancer therapy. These novel applications and techniques include: Nanogels for loading siRNA. this can be atiny low officious RNA (siRNA) may be a category of double-stranded RNA molecules consisting of 21–23 nucleotides, concerned in inhibition of supermolecule synthesis encoded by the traveler RNAs.