Targeted Treatment Approaches in Lung Cancer Based on Molecular Profiling

Sierra Ximena^*
*Correspondence: Sierra Ximena, Department of Clinical and Experimental Medicine, University of Modena and Reggio Emilia, 41121 Modena, Italy, Email:

Introduction

Lung cancer remains one of the leading causes of cancer-related deaths worldwide. Traditional treatment strategies, including surgery, chemotherapy, and radiation, have been effective to some extent but are often limited by issues such as drug resistance, side effects, and the heterogeneous nature of lung cancer. In recent years, molecular profiling has emerged as a revolutionary approach, enabling more personalized and targeted therapies for lung cancer patients. By analyzing the genetic mutations, alterations, and molecular markers present in a patient's tumor, doctors can tailor treatments that are more effective and have fewer side effects. This article explores how molecular profiling is reshaping lung cancer treatment through targeted approaches. Lung cancer is one of the most prevalent and deadly forms of cancer worldwide, accounting for a significant number of cancer-related deaths each year. It primarily arises from the tissues of the lungs, often due to prolonged exposure to risk factors such as smoking, environmental pollutants, and genetic predispositions. Lung cancer remains one of the leading causes of cancer-related mortality worldwide. It is a heterogeneous disease with various histopathological and molecular subtypes, each having distinct clinical and biological characteristics. Understanding these subtypes is crucial for accurate diagnosis, effective treatment and the development of targeted therapies. This article provides a comprehensive overview of the histopathological and molecular profiling of bronchial cancer subtypes, focusing on their diagnostic features, molecular alterations and clinical implications [1].

Description

Molecular profiling refers to the process of examining a cancer’s genetic makeup, including the identification of specific mutations, alterations in gene expression, and protein expression patterns. In lung cancer, this profiling often focuses on mutations in key genes that drive tumor growth, such as EGFR (epidermal growth factor receptor), KRAS, ALK (anaplastic lymphoma kinase), and ROS1. Advancements in molecular characterization have significantly enhanced our understanding of lung cancer at the genetic and biochemical levels. This has paved the way for targeted therapies that aim to specifically inhibit cancer cell growth, offering more personalized treatment options. On-going research continues to explore new biomarkers and therapeutic strategies, highlighting the importance of early detection and innovative treatment approaches in improving patient outcomes. Bronchial cancer is broadly classified into two major categories: Non-Small Cell Bronchial Cancer (NSCBC) and Small Cell Bronchial Cancer (SCBC).

Adenocarcinoma, this is the most common subtype, representing about 40% of bronchial cancers. It arises from the glandular cells of the lung and is often found in the outer regions of the lung. Histologically, adenocarcinomas can be identified by glandular formation and cumin production. Subtypes include cigar, papillary, micro papillary and solid adenocarcinomas. Squamous Cell Carcinoma (SCC), accounting for about 25-30% of bronchial cancers, SCC arises from the squamous cells lining the airways. It typically occurs in the central part of the lungs. Histopathological features include keratinization and intercellular bridges. SCC is associated with smoking and tends to present with necrosis and cavitation. Large Cell Carcinoma, this subtype constitutes about 10-15% of bronchial cancers and is characterized by large, undifferentiated cells. It can occur in any part of the lung and lacks the glandular or squamous differentiation seen in adenocarcinoma and SCC [2].

Lung cancer is primarily divided into two types: non-small cell lung cancer (NSCLC), which accounts for approximately 85% of cases, and small cell lung cancer (SCLC). Molecular profiling is particularly relevant for NSCLC, where genetic mutations and alterations in signaling pathways can guide therapeutic decisions. SCLC accounts for approximately 15% of bronchial cancers and is strongly associated with smoking. It is an aggressive form of bronchial cancer, often presenting with rapid growth and early metastasis. Histologically, SCLC is characterized by small, round to oval cells with scant cytoplasm, finely granular chromatin and a high mitotic rate. It often exhibits neuroendocrine features and expresses markers such as synaptophysin, chromogranin and CD56. Molecular profiling has revolutionized the understanding and treatment of bronchial cancer by identifying specific genetic alterations that drive tumour genesis. These alterations serve as targets for personalized therapies and include mutations, gene rearrangements and amplifications.

Epidermal Growth Factor Receptor (EGFR) mutations are prevalent in approximately 10-15% of NSCLC cases, particularly in adenocarcinomas and non-smokers. Common mutations occur in exons 18-21, leading to constitutive activation of the EGFR pathway. Targeted therapies, such as Tyrosine Kinase Inhibitors (TKIs), have shown significant efficacy in patients with EGFR-mutant NSCLC. KRAS mutations are found in about 25-30% of NSCLC cases, primarily in adenocarcinomas and smokers. These mutations lead to the activation of the RAS/MAPK pathway. Unlike EGFR, targeted therapies for KRAS-mutant NSCLC have been challenging, but recent developments, such as KRAS G12C inhibitors, offer new treatment options. Anaplastic Lymphoma Kinase (ALK) rearrangements occur in approximately 3-5% of NSCLC cases. The EML4-ALK fusion gene results in constitutive kinase activity. ALK inhibitors, such as crizotinib and alectinib, have demonstrated substantial clinical benefits in ALK-positive NSCLC patients. ROS1 gene rearrangements are present in about 1-2% of NSCLC cases. Similar to ALK, ROS1 fusions lead to constitutive kinase activation. ROS1 inhibitors, such as crizotinib, are effective in treating ROS1-positive NSCLC. BRAF mutations, particularly V600E, are found in about 1-2% of NSCLC cases. These mutations activate the MAPK pathway. BRAF inhibitors, either alone or in combination with MEK inhibitors, provide therapeutic options for BRAF-mutant NSCLC. MET exon 14 skipping mutations occur in approximately 3-4% of NSCLC cases. These mutations lead to the activation of the MET pathway. MET inhibitors, such as capmatinib and tepotinib, are effective in patients with MET exon 14 alterations. SCLC is characterized by frequent alterations in tumour suppressor genes, such as TP53 and RB1. TP53 mutations are present in nearly all SCLC cases, leading to the loss of p53 tumour suppressor function. This contributes to the high genomic instability and aggressive nature of SCLC [3].

The integration of histopathological and molecular profiling into clinical practice has significant implications for the management of bronchial cancer: The identification of specific molecular alterations has enabled the development of targeted therapies, improving treatment outcomes and reducing toxicity. Examples include EGFR TKIs, ALK inhibitors and ROS1 inhibitors. Molecular profiling allows for the customization of treatment plans based on the individual genetic makeup of the tumour. This approach maximizes therapeutic efficacy and minimizes adverse effects. Certain molecular alterations serve as prognostic biomarkers, providing information on disease progression and survival outcomes. For instance, EGFR mutations are associated with better response rates to TKIs. Molecular alterations can predict the likelihood of response to specific therapies. For example, ALK rearrangements predict responsiveness to ALK inhibitors. Molecular profiling can aid in the early detection and screening of bronchial cancer, particularly in high-risk populations. Liquid biopsies, which analyse circulating tumour DNA, offer a non-invasive method for monitoring molecular changes. The integration of histopathological and molecular profiling is essential for accurate diagnosis and personalized treatment. Histopathological examination provides initial insights into the subtype of bronchial cancer, guiding subsequent molecular testing. Molecular profiling then identifies specific genetic alterations, enabling tailored therapeutic approaches. This combined approach enhances the precision of bronchial cancer management, leading to better prognostic outcomes and improved survival rates [4,5].

Conclusion

Molecular profiling has fundamentally changed the landscape of lung cancer treatment by enabling personalized and targeted therapies based on specific genetic mutations and molecular markers. This approach improves treatment efficacy, reduces side effects, and offers new hope for patients with advanced disease. As new molecular targets and therapies continue to emerge, lung cancer treatment will become even more precise and tailored to individual patients. The integration of molecular profiling with liquid biopsy and immunotherapy holds significant promise for the future of lung cancer management, moving closer to a more effective and personalized approach to treatment.

Acknowledgment

None.

Conflict of Interest

None.

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