Vibrational Spectroscopy for Bio-Active Compound Detection in Virgin Olive Oils: Recent Advances and Reviews

Wanchat Zeouk^*
*Correspondence: Wanchat Zeouk, Department of Food Technology,, University of Milan, Italy, Email:

Introduction

Virgin Olive Oil (VOO) is a highly valued natural product known for its numerous health benefits, attributed to its bio-active compounds such as polyphenols, phenolic acids, flavonoids, and tocopherols. These bio-active compounds are responsible for the antioxidant, anti-inflammatory, and cardiovascular protective properties of olive oil, making it a key component of the Mediterranean diet. However, the composition and quality of VOO can be influenced by various factors, including the olive variety, geographic origin, and extraction methods. Therefore, there is a growing need for reliable, non-invasive methods to detect and quantify these bio-active compounds in olive oil, ensuring both quality control and authenticity. Vibrational spectroscopy techniques, such as Fourier-Transform Infrared (FTIR) spectroscopy and Raman spectroscopy, have emerged as powerful analytical tools for detecting bio-active compounds in VOO. These techniques offer high sensitivity, minimal sample preparation, and the ability to provide detailed molecular information about the chemical composition of olive oil. Recent advancements in vibrational spectroscopy have further improved its applicability for the rapid, on-site detection of bio-active compounds in virgin olive oils, leading to more efficient and accurate quality assessments. [1] Recent reviews of vibrational spectroscopy applications in food quality assessment highlight the growing interest in utilizing these methods for the detection of bio-active compounds in VOO. FTIR and Raman spectroscopy, in particular, offer several advantages over traditional chemical analyses, such as high throughput, non-destructive testing, and the ability to perform real-time analyses. These techniques rely on the interaction of light with matter to produce spectra that contain unique information about the molecular vibrations of different functional groups present in a sample. In VOO, the presence of specific functional groups related to bio-active compounds, such as hydroxyl, carbonyl, and aromatic groups, can be detected and quantified. Moreover, the development of portable and miniaturized spectroscopic instruments has enabled the use of these techniques in quality control during olive oil production and distribution. As the demand for high-quality olive oil continues to rise globally, the application of vibrational spectroscopy in detecting bio-active compounds promises to enhance quality assurance practices and safeguard the authenticity of VOO on the market. [2]

Description

Fourier-Transform Infrared (FTIR) spectroscopy is one of the most widely used vibrational spectroscopy techniques for analyzing the chemical composition of virgin olive oil. FTIR works by measuring the absorption of infrared light at different wavelengths, corresponding to the vibrational frequencies of various molecular bonds in the sample. The resulting spectrum provides a unique fingerprint of the chemical composition of the sample, enabling the identification and quantification of bio-active compounds in VOO. In recent years, FTIR has been successfully applied to detect phenolic compounds, tocopherols, and other antioxidants in virgin olive oils, which are responsible for its health benefits. Moreover, advancements in data analysis techniques, such as chemometrics, have enhanced the sensitivity and accuracy of FTIR in differentiating between various olive oil samples based on their bio-active compound content. This has made FTIR a valuable tool for ensuring the quality and authenticity of VOO, as well as for monitoring the impact of production processes on its bioactive content. FTIR spectroscopy is also relatively quick and cost-effective, making it an ideal method for large-scale quality control and monitoring.

Raman spectroscopy, another key vibrational technique, has also gained attention in recent years for detecting bio-active compounds in virgin olive oil. Raman spectroscopy relies on the inelastic scattering of light, which provides information about the vibrational modes of molecules. In the case of VOO, Raman spectroscopy is particularly useful for identifying polyphenols, oleic acid, and other bioactive lipids. Unlike FTIR, which measures absorption, Raman spectroscopy measures the scattering of light, making it particularly effective for analyzing non-polar compounds, such as lipids, that are abundant in olive oil. Raman spectra are highly informative and can be used to detect subtle variations in the composition of olive oil, such as changes in phenolic content or oxidative degradation. One of the key advantages of Raman spectroscopy is its ability to provide molecular-level insights without the need for extensive sample preparation, making it ideal for on-site analysis of VOO quality during production or distribution. The integration of Raman spectroscopy with other techniques, such as multivariate analysis, has further enhanced its utility in detecting bio-active compounds and distinguishing between different grades and varieties of olive oil.

The application of vibrational spectroscopy in bio-active compound detection in virgin olive oils has also seen the development of portable and miniaturized devices that offer significant advantages for on-site analysis. These portable spectrometers are equipped with advanced sensors and software that allow for real-time analysis of olive oil samples in the field, without the need for sample extraction or extensive laboratory processing. This innovation has opened new possibilities for rapid quality assessment in the olive oil industry, especially in regions where traditional laboratory testing may not be feasible. Furthermore, the combination of vibrational spectroscopy with other analytical methods, such as Gas Chromatography-Mass Spectrometry (GC-MS) or High-Performance Liquid Chromatography (HPLC), has allowed for a more comprehensive analysis of VOO, providing complementary information about its chemical composition. The synergistic use of these techniques enhances the accuracy and reliability of bio-active compound detection, offering more robust quality assurance and ensuring the integrity of virgin olive oils in the market.

Conclusion

In conclusion, vibrational spectroscopy has emerged as a powerful tool for the detection and quantification of bio-active compounds in virgin olive oils. Both FTIR and Raman spectroscopy have proven to be effective methods for analyzing the chemical composition of olive oil, offering high sensitivity, non-destructive testing, and the ability to provide detailed molecular information about bio-active compounds. Recent advances in spectroscopic technologies, including the development of portable and miniaturized devices, have further enhanced the practicality and accessibility of these techniques for on-site quality control. By accurately detecting phenolic compounds, antioxidants, and other bio-active molecules, vibrational spectroscopy helps ensure the authenticity and quality of virgin olive oil, benefiting producers, distributors, and consumers alike. Additionally, the integration of these techniques with chemometrics and multivariate analysis allows for more precise and comprehensive assessments of olive oil composition, leading to better quality assurance practices in the olive oil industry. As demand for high-quality, authentic virgin olive oils continues to rise, the application of vibrational spectroscopy techniques will play an increasingly important role in safeguarding the integrity and health benefits of this valuable product. Moving forward, continued advancements in vibrational spectroscopy, coupled with innovations in data analysis, promise to further enhance the accuracy and efficiency of bio-active compound detection in virgin olive oils.

References

1. Raymond, Elizabeth G., Margo S. Harrison and Mark A. Weaver. "Efficacy of misoprostol alone for first-trimester medical abortion: a systematic review." Gynecol 133 (2019): 137-147.

Google Scholar, Crossref, Indexed at

2. Summers, P. M., Caroline J. Wennink and J. K. Hodges. "Cloprostenol-induced luteolysis in the marmoset monkey (Callithrix jacchus)." J Reprod Fertil 73 (1985): 133-138.

Google Scholar, Crossref, Indexed at