Zhao Da-qiang, Li Si-wen, Liao Tao, Huang Zheng-yu, Han Fei, Hua Xue-feng, Li Heng and Sun Qi-quan
Third Affiliated Hospital of Sun Yat-sen University, China
Posters & Accepted Abstracts: J Nephrol Ther
6-8 weeks old male C3H and Balb/c mice were used as skin or kidney graft donors and recipients. Donor specific antibody (DSA) levels of recepient serum were monitored within 100 days post skin presensitization. Recipient animals were divided into three groups: The ever reported method group (ST-7dTx) and improved method groups (ST-4dTx, ST-3dTx). Recipient animals were presensitized by transplanting skin grafts before seven, four and three days of kidney transplantation in ST- 7dTx, ST-4dTx and ST-3dTx groups. Animal survival time was recorded. The diagnosis of renal antibody mediated rejection (AMR) was evaluated based on Banff 2013 criteria. DSA-IgG level continously elevated within 50 days after presensitized by skin transplantation and kept a plateau within 50-100 days. DSA-IgM level did not incease remarkably within 100 days post skin presensitization. The median survival time of ST-7dTx, ST-4dTx, and ST-3dTx groups were four, seven and nine days. All recepient animals in ST-7dTx and ST-4dTx groups died within 14 days post kidney transplantation. 17% recepients in ST-3dTx acquired long-term survival (>60d). The dominant death reason for all animals was acute AMR of renal grafts, which met Banff 2013 criteria. Recepient animals in improved models survived and allowed longer time for intervening AMR. So, the improved model is superior to the ever reported one. Some recepient animals in ST-3dTx group can survive, which may interfer the observation results when applying the model to evaluating the effects of therapeutic strategies for renal graft AMR, so ST-4d Tx is a more idea murine acute AMR kidney transplantation model.
Email: zhaodaq@mail.sysu.edu.cn
Journal of Nephrology & Therapeutics received 784 citations as per Google Scholar report
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